Gutzeit H O, Eberhardt W, Gratwohl E
Institut für Biologie I, Albert-Ludwigs-Universität Freiburg, Germany.
J Cell Sci. 1991 Dec;100 ( Pt 4):781-8. doi: 10.1242/jcs.100.4.781.
The localization of the extracellular matrix glycoprotein laminin was studied using polyclonal anti-laminin antibodies. The laminin patterns of the basement membranes of the muscular epithelial sheath that envelops the ovariole were conspicuously different from those of the basement membrane of the follicular epithelium. In the latter structure laminin was stained in a pattern of parallel stripes oriented perpendicular to the long axis of the follicle; microfilament bundles at the adjacent basal side of the follicle cells have the same orientation. At late vitellogenic stages the orientation of the microfilaments remained the same while the laminin stripes were no longer visible. The orientation of laminin and F-actin was abnormal in follicles of the egg-shape mutant kugel, which produces shorter and thicker eggs than wild-type flies. This phenotype might result from the disturbance of the normal circular microfilament and/or laminin pattern.
利用多克隆抗层粘连蛋白抗体研究了细胞外基质糖蛋白层粘连蛋白的定位。包裹卵巢管的肌上皮鞘基底膜的层粘连蛋白模式与卵泡上皮基底膜的层粘连蛋白模式明显不同。在后者结构中，层粘连蛋白以垂直于卵泡长轴的平行条纹模式染色；卵泡细胞相邻基底侧的微丝束具有相同的方向。在卵黄发生后期，微丝的方向保持不变，而层粘连蛋白条纹不再可见。在卵形突变体kugel的卵泡中，层粘连蛋白和F-肌动蛋白的方向异常，该突变体产生的卵比野生型果蝇的卵更短更厚。这种表型可能是由于正常圆形微丝和/或层粘连蛋白模式受到干扰所致。
