Gutzeit H O
Institut für Biologie I, Albert-Ludwigs-Universität, Freiburg/Federal Republic of Germany.
Eur J Cell Biol. 1990 Dec;53(2):349-56.
The microfilament pattern in the somatic follicle cells of mid-vitellogenic stage 9 to 11 follicles of Drosophila was analyzed by staining F-actin with fluorescence-labeled phalloidin. During the analyzed stages of oogenesis, the follicular epithelium differentiates morphologically and functionally. These changes are also reflected at the organization of the microfilaments. At stage 10, they show no preferred orientation in the very thin follicle cells covering the nurse cells. In contrast, the microfilaments in the basal part of the columnar follicle cells covering the oocyte become organized in parallel bundles oriented perpendicular to the long axis of the follicle. During stages 10B/11 this organization is maintained at the nurse cell/oocyte border but becomes more sloppy towards the posterior pole of the follicle. The basal part of the follicle cells containing the microfilament bundles adheres so tightly to the basement membrane that this acellular layer cannot be separated mechanically from the epithelium. Indirect evidence from inhibition studies with cytochalasins and the effects of collagenase or pronase E added to the culture medium suggest that the microfilament bundles may promote increased adhesiveness of the follicle cells to the basement membrane. The possible functional implications of the microfilaments and their orientation are discussed.
通过用荧光标记的鬼笔环肽对F-肌动蛋白进行染色，分析了果蝇9至11期卵黄发生中期卵泡的体细胞卵泡细胞中的微丝模式。在分析的卵子发生阶段，卵泡上皮在形态和功能上发生分化。这些变化也反映在微丝的组织上。在第10阶段，覆盖滋养细胞的非常薄的卵泡细胞中，微丝没有明显的取向。相反，覆盖卵母细胞的柱状卵泡细胞基部的微丝排列成与卵泡长轴垂直的平行束。在10B/11阶段，这种排列在滋养细胞/卵母细胞边界处保持，但在卵泡后极方向变得更加不规则。含有微丝束的卵泡细胞基部与基底膜紧密粘附，以至于这个无细胞层不能从上皮机械分离。用细胞松弛素进行的抑制研究以及添加到培养基中的胶原酶或链霉蛋白酶E的作用的间接证据表明，微丝束可能促进卵泡细胞与基底膜的粘附增加。讨论了微丝及其取向可能的功能意义。
