前列环素和卡巴前列环素对培养的血管平滑肌细胞中内皮素诱导的DNA合成的抑制作用。

Inhibition by prostacyclin and carbacyclins of endothelin-induced DNA synthesis in cultured vascular smooth muscle cells.

作者信息

Nakaki T, Ohta M, Kato R

机构信息

Department of Pharmacology, Keio University School of Medicine, Tokyo, Japan.

出版信息

Prostaglandins Leukot Essent Fatty Acids. 1991 Dec;44(4):237-9. doi: 10.1016/0952-3278(91)90023-x.

DOI:10.1016/0952-3278(91)90023-x

PMID:1815238

Abstract

Effects of prostacyclin and carbacyclins on endothelin-induced DNA synthesis were investigated in vascular smooth muscle cells. DNA synthesis was estimated by [3H]thymidine incorporation. Five carbacyclins used in this report were 5-[(1S, 5S, 6R, 7R)-7-hydroxy-6-[(E)-(S)-3-hydroxy-1-octenyl]bicyclo [3.3.0]oct-2-en-3-yl) pentanoic acid (TEI-7165), methyl 5-[(1S, 5S, 6R, 7R)-7-hydroxy-6-[(E)-(S)-3-hydroxy-1-octenyl]bicyclo[3.3.0]oct-2-en-3- yl]pentanoate (TEI-9090), 5-[(1S, 5S, 6R, 7R)-7-hydroxy-6-[(E)-(3S, 5S)-3-hydroxy-5-methyl-1-nonenyl]bicyclo[3.3.0]oct-2-en-3-yl)penta noic acid (TEI-9063), methyl 5-[(1S, 5S, 6R, 7R)-7-hydroxy-6-[(E)-(3S, 5S)-3-hydroxy-5-methyl-1- nonenyl]bicyclo[3.3.0]oct-2-en-3-yl)pentanoate (TEI-1324), 5-[(1S, 5S, 6R, 7R)-7-hydroxy-6-[(E)-(S)-4-hydroxy-4-methyl-1- octenyl]bicyclo[3.3.0]oct-2-en-3-yl] pentanoic acid (TEI-3356). Prostacyclin and the carbacyclins inhibited the endothelin-induced DNA synthesis within the nanomolar range. These results suggest that prostacyclin and carbacyclins are possibly effective in inhibiting the proliferation of vascular smooth muscle cells under some situations in vivo.

摘要

在血管平滑肌细胞中研究了前列环素和环前列素对内皮素诱导的DNA合成的影响。通过[³H]胸苷掺入来估计DNA合成。本报告中使用的五种环前列素分别是5-[(1S, 5S, 6R, 7R)-7-羟基-6-[(E)-(S)-3-羟基-1-辛烯基]双环[3.3.0]辛-2-烯-3-基]戊酸（TEI-7165）、5-[(1S, 5S, 6R, 7R)-7-羟基-6-[(E)-(S)-3-羟基-1-辛烯基]双环[3.3.0]辛-2-烯-3-基]戊酸甲酯（TEI-9090）、5-[(1S, 5S, 6R, 7R)-7-羟基-6-[(E)-(3S, 5S)-3-羟基-5-甲基-1-壬烯基]双环[3.3.0]辛-2-烯-3-基]戊酸（TEI-9063）、5-[((1S, 5S, 6R, 7R)-7-羟基-6-[(E)-(3S, 5S)-3-羟基-5-甲基-1-壬烯基]双环[3.3.0]辛-2-烯-3-基]戊酸甲酯（TEI-1324）、5-[(1S, 5S, 6R, 7R)-7-羟基-6-[(E)-(S)-4-羟基-4-甲基-1-辛烯基]双环[3.3.0]辛-2-烯-3-基]戊酸（TEI-3356）。前列环素和环前列素在纳摩尔范围内抑制内皮素诱导的DNA合成。这些结果表明，在体内某些情况下，前列环素和环前列素可能有效地抑制血管平滑肌细胞的增殖。