de Guzman A M, de Pederiva N B, Eiguer T, Caffer M I, Fronchkowsky B
Cátedra de Bacteriología I, Facultad de Química, Universidad Nacional de San Luis, Argentina.
Rev Argent Microbiol. 1991 Jan-Feb;23(1):35-40.
Yersinia was investigated in 50 skin samples of chicken carcasses from retail shops and 65 samples of balanced food for domestic fowl. Enrichments were performed in saline phosphate buffer 0.067 M, pH 7.6 and post-enriched in 0.5% KOH. Subcultures were performed in Salmonella-Shigella agar and MacConkey agar. Isolates were identified through biochemical, serological and lysotyping methods. The following biovar (B), serovar (O) and phagovar (Lis) were isolated from chickens: Y. enterocolitica (five strains) B:1:O:6,47;Lis Xz; B:1;O:6:Lis Xz; B:1:O:5,Lis Xz; Y.intermedia (two strains) B:1;O:52;Lis Xz; B:1;O:52,53,54;Lis Xz (NRA, nitrate reductase type A); Y. frederiksenii (two strains) O:10,K1,25,35,38,46:Lis Xz; (citrate): O:10,K1,25,35,38,46:Lis Xz (ONPG-: citrate +); Y. kristensenii (one strain) does not agglutinate; Lis Xo. Yersinia were not isolated from balanced food for domestic fowl. Virulence tests (calcium dependency and autoagglutination at 37 degrees C) were negative in all instances. It is concluded from this study that Yersinia isolated from chickens are without pathogenic importance.
对来自零售店的50份鸡 carcasses 皮肤样本和65份家禽均衡食品样本进行了耶尔森氏菌调查。在0.067M、pH 7.6的磷酸盐缓冲盐水中进行富集培养,并在0.5%氢氧化钾中进行后富集培养。在沙门氏菌-志贺氏菌琼脂和麦康凯琼脂上进行传代培养。通过生化、血清学和噬菌体分型方法对分离株进行鉴定。从鸡中分离出以下生物型(B)、血清型(O)和噬菌体型(Lis):小肠结肠炎耶尔森氏菌(5株)B:1:O:6,47;Lis Xz;B:1;O:6:Lis Xz;B:1:O:5,Lis Xz;中间耶尔森氏菌(2株)B:1;O:52;Lis Xz;B:1;O:52,53,54;Lis Xz(NRA,A型硝酸盐还原酶);费氏耶尔森氏菌(2株)O:10,K1,25,35,38,46:Lis Xz;(柠檬酸盐):O:10,K1,25,35,38,46:Lis Xz(ONPG-:柠檬酸盐+);克氏耶尔森氏菌(1株)不凝集;Lis Xo。在家禽均衡食品中未分离出耶尔森氏菌。所有情况下的毒力试验(钙依赖性和37摄氏度下的自凝)均为阴性。从这项研究得出结论,从鸡中分离出的耶尔森氏菌没有致病重要性。 (注:carcasses这个词在原文中可能有误,推测可能是carcass,意为“屠体” )