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马鞭草和柠檬马鞭草水制剂成分的高效液相色谱-二极管阵列检测-电喷雾电离质谱分析及抗氧化活性评价

HPLC-DAD-ESI-MS analysis of the constituents of aqueous preparations of verbena and lemon verbena and evaluation of the antioxidant activity.

作者信息

Bilia A R, Giomi M, Innocenti M, Gallori S, Vincieri F F

机构信息

Department of Pharmaceutical Sciences, University of Florence, Via Ugo Schiff 6, 50019 Sesto Fiorentino, (FI), Italy.

出版信息

J Pharm Biomed Anal. 2008 Feb 13;46(3):463-70. doi: 10.1016/j.jpba.2007.11.007. Epub 2007 Nov 17.

DOI:10.1016/j.jpba.2007.11.007
PMID:18155378
Abstract

Verbena and lemon verbena aqueous preparations were investigated for their content of constituents, especially polyphenols by HPLC/DAD/ESI/MS analysis because they are used worldwide as herbal teas. The main class of compounds of these plants were phenylpropanoids (from 16 to 120 mg/g of dried extract), being verbascoside the most abundant in all the preparations up to 97% of the total phenylpropanoids. Also iridoids, hastatoside and verbenalin together with flavonoids, mono- and di-glucuronidic derivatives of luteolin and apigenin were found. These simple preparations, especially that obtained from infusion of lemon verbena, could be lyophilized to obtain a powder having interesting technological properties to be used as ingredients of cosmetics, food supplements and herbal medicinal products do to the many biological properties of verbascoside. In addition, the antioxidant property of the lemon verbena infusion was evaluated by the DPPH test using Trolox as the reference compound.

摘要

马鞭草和柠檬马鞭草的水制剂因其成分含量,特别是通过HPLC/DAD/ESI/MS分析其多酚含量而受到研究,因为它们在全球范围内被用作花草茶。这些植物的主要化合物类别是苯丙素类(每克干提取物含16至120毫克),毛蕊花糖苷在所有制剂中含量最高,占总苯丙素类的97%。还发现了环烯醚萜类、哈斯塔糖苷和马鞭草苷以及黄酮类化合物,木犀草素和芹菜素的单葡萄糖醛酸和双葡萄糖醛酸衍生物。这些简单的制剂,特别是从柠檬马鞭草浸液中获得的制剂,可以冻干得到具有有趣技术特性的粉末,由于毛蕊花糖苷具有多种生物学特性,可作为化妆品、食品补充剂和草药产品的成分。此外,以Trolox作为参考化合物,通过DPPH试验评估了柠檬马鞭草浸液的抗氧化性能。

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