Laboratoire de Chimie Agro-industrielle (LCA), Université de Toulouse, INRAE, INPT, 4 Allée Emile Monso, 31030 Toulouse, France.
Mycoscopia, 3 rue Jean Monnet, 31470 Fonsorbes, France.
Molecules. 2023 Jun 30;28(13):5123. doi: 10.3390/molecules28135123.
Aflatoxin B1 (AFB1) is a mycotoxin considered a potent carcinogen for humans that contaminates a wide range of crops. Various strategies have been established to reduce or block the synthesis of AFB1 in food and feed. The use of aqueous extracts derived from plants with high antioxidant activity has been a subject of study in recent years due to their efficacy in inhibiting AFB1. In this study, we assessed the effect of aqueous extract on growth and on AFB1 production. A bio-guided fractionation followed by High Performance Liquid Chromatography (HPLC) and Mass spectrometry analysis of the active fraction were applied to identify the candidate molecules responsible for the dose-effect inhibition of AFB1 synthesis. Our results revealed that polyphenols are the molecules implicated in AFB1 inhibition, achieving almost a total inhibition of the toxin production (99%). We identified luteolin-7-diglucuronide as one of the main constituents in extract, and demonstrated that it is able to inhibit, by itself, AFB1 production by 57%. This is the first study demonstrating the anti-Aflatoxin B1 effect of this molecule, while other polyphenols surely intervene in anti-AFB1 activity.
黄曲霉毒素 B1（AFB1）是一种被认为对人类有很强致癌性的真菌毒素，会污染多种农作物。人们已经制定了各种策略来减少或阻断食品和饲料中 AFB1 的合成。近年来，由于其抑制 AFB1 的功效，从具有高抗氧化活性的植物中提取的水提取物已成为研究的主题。在这项研究中，我们评估了水提取物对生长和 AFB1 产生的影响。采用生物导向的分级分离，然后对活性部分进行高效液相色谱（HPLC）和质谱分析，以鉴定负责抑制 AFB1 合成的候选分子。我们的结果表明，多酚是抑制 AFB1 的分子，几乎完全抑制了毒素的产生（99%）。我们鉴定出叶黄素-7-二葡萄糖苷是提取物中的主要成分之一，并证明它本身就能抑制 AFB1 的产生，抑制率为 57%。这是首次证明该分子具有抗黄曲霉毒素 B1 作用的研究，而其他多酚肯定也参与了抗 AFB1 活性。
