高效液相色谱-二极管阵列检测器-电喷雾串联质谱法同时测定水飞蓟和丹参复方制剂中的枞酸型二萜、黄酮木脂素及酚类化合物

Simultaneous determination of abietane-type diterpenes, flavonolignans and phenolic compounds in compound preparations of Silybum marianum and Salvia miltiorrhiza by HPLC-DAD-ESI MS.

作者信息

Zhao Yu, Chen Bo, Yao Shouzhuo

机构信息

Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, China.

出版信息

J Pharm Biomed Anal. 2005 Jul 1;38(3):564-70. doi: 10.1016/j.jpba.2005.01.021. Epub 2005 Feb 26.

DOI:10.1016/j.jpba.2005.01.021

PMID:15925261

Abstract

A gradient HPLC-DAD-ESI MS method has been developed for simultaneous determination of multiple bioactive compounds such as abietane-type diterpenes, flavonolignans and phenolic compounds in compound preparations of Silybum marianum and Salvia miltiorrhiza. The separation was completed on an ODS column using 0.5% (v/v) formic acid aqueous solution and methanol as gradient mobiles. Fourteen components can be identified by ESI MS working on ESI(-) and ESI(+) switching mode, respectively. Ten components can be quantified by using external standard method with UV detecting at 254 and 280 nm, respectively. The correlation coefficients of all the calibration curves were found to be higher than 0.992. The recoveries of the standards were about 96-101%. Besides quantification of the components, the chromatograms acquired by this method can be used as the bioactive components fingerprints for the quality control of compound preparations of S. marianum and S. miltiorrhiza.

摘要

建立了一种梯度高效液相色谱-二极管阵列检测器-电喷雾电离质谱法，用于同时测定水飞蓟和丹参复方制剂中的多种生物活性化合物，如松香烷型二萜、黄酮木脂素和酚类化合物。分离在ODS柱上进行，以0.5%（v/v）甲酸水溶液和甲醇作为梯度流动相。通过分别在电喷雾电离质谱的ESI（-）和ESI（+）切换模式下工作，可以鉴定出14种成分。通过外标法，分别在254和280 nm处进行紫外检测，可以对10种成分进行定量。所有校准曲线的相关系数均高于0.992。标准品的回收率约为96%-101%。除了对成分进行定量外，该方法获得的色谱图还可作为水飞蓟和丹参复方制剂质量控制的生物活性成分指纹图谱。