Piletska Elena V, Guerreiro Antonio R, Romero-Guerra Maria, Chianella Iva, Turner Anthony P F, Piletsky Sergey A
Cranfield Health, Cranfield University, Silsoe, Bedfordshire MK45 4DT, UK.
Anal Chim Acta. 2008 Jan 21;607(1):54-60. doi: 10.1016/j.aca.2007.11.019. Epub 2007 Nov 19.
The main problem of poor water compatibility of molecularly imprinted polymers (MIPs) was addressed in examples describing design of synthetic receptors with high affinity for drugs of abuse. An extensive potentiometric titration of 10 popular functional monomers and corresponding imprinted and blank polymers was conducted in order to evaluate the subtleties of functional groups ionisation under aqueous conditions. It was found that polymers prepared using 2-trifluoromethacrylic acid (TFMAA) in combination with toluene as porogen possess superior properties which make them suitable for effective template recognition in water. The potential impact of phase separation during polymerisation on formation of high quality imprints has been discussed. Three drugs of abuse such as cocaine, deoxyephedrine and methadone were used as template models in polymer preparation for the practical validation of obtained results. The polymer testing showed that synthesized molecularly imprinted polymers have high affinity and selectivity for corresponding templates in aqueous environment, with imprinting factors of 2.6 for cocaine and 1.4 for methadone and deoxyephedrine. Corresponding blank polymers were unable to differentiate between analytes, suggesting that imprinting phenomenon was responsible for the recognition properties.
在描述对滥用药物具有高亲和力的合成受体设计的实例中，解决了分子印迹聚合物（MIPs）水相容性差的主要问题。为了评估水性条件下官能团电离的细微差别，对10种常用功能单体以及相应的印迹聚合物和空白聚合物进行了广泛的电位滴定。结果发现，使用2-三氟甲基丙烯酸（TFMAA）与甲苯作为致孔剂制备的聚合物具有优异的性能，使其适用于在水中进行有效的模板识别。讨论了聚合过程中相分离对高质量印迹形成的潜在影响。在聚合物制备过程中，使用可卡因、脱氧麻黄碱和美沙酮这三种滥用药物作为模板模型，以实际验证所得结果。聚合物测试表明，合成的分子印迹聚合物在水性环境中对相应模板具有高亲和力和选择性，可卡因的印迹因子为2.6，美沙酮和脱氧麻黄碱的印迹因子为1.4。相应的空白聚合物无法区分分析物，这表明印迹现象是识别特性的原因。
