Athikomrattanakul Umporn, Katterle Martin, Gajovic-Eichelmann Nenad, Scheller Frieder W
Fraunhofer Institute for Biomedical Engineering, Am Muehlenberg 13, Potsdam 14476, Germany.
Biosens Bioelectron. 2009 Sep 15;25(1):82-7. doi: 10.1016/j.bios.2009.06.003. Epub 2009 Jun 10.
Novel molecularly imprinted polymers (MIPs) for the recognition of nitrofurantoin (NFT) were prepared by photoinitiated polymerisation in polar solvent using 2,6-bis(methacrylamido) pyridine (BMP) as the functional monomer and carboxyphenyl aminohydantoin (CPAH) as the analogue of the template. The binding constants of the complex between BMP and nitrofurantoin or CPAH in DMSO were determined with 1H NMR titration to be 630+/-104 and 830+/-146 M(-1), respectively. To study the influence of the functional monomer, two polymer compositions were prepared containing the template, the functional monomer and the crosslinker in the molar ratio 1:1:12 for MIP1 and 1:4:20 for MIP2, respectively. The imprinting factor at saturation concentration of nitrofurantoin, which is the ratio of the amount bound to the MIP and the non-imprinted control polymer (NIP), was determined to be 2.47 for MIP1 and 2.49 for MIP2. The cross reactivity of the imprinted polymers seems to be determined by the ability to form hydrogen bonds to the functional monomer while the shape of the molecule has no real influence.
采用光引发聚合方法，以2,6 - 双（甲基丙烯酰胺基）吡啶（BMP）为功能单体、羧基苯基氨基乙内酰脲（CPAH）为模板类似物，在极性溶剂中制备了用于识别呋喃妥因（NFT）的新型分子印迹聚合物（MIPs）。通过¹H NMR滴定法测定了BMP与呋喃妥因或CPAH在二甲基亚砜（DMSO）中的配合物结合常数，分别为630±104 M⁻¹和830±146 M⁻¹。为研究功能单体的影响，制备了两种聚合物组合物，其中模板、功能单体和交联剂的摩尔比对于MIP1为1:1:12，对于MIP2为1:4:20。在呋喃妥因饱和浓度下的印迹因子，即结合到MIP上的量与非印迹对照聚合物（NIP）的量之比，对于MIP1测定为2.47，对于MIP2测定为2.49。印迹聚合物的交叉反应性似乎由与功能单体形成氢键的能力决定，而分子形状没有实际影响。
