Department of Biotechnology, Aalborg University, DK-9000 Aalborg, Denmark.
Biosens Bioelectron. 2009 Nov 15;25(3):623-8. doi: 10.1016/j.bios.2009.01.021. Epub 2009 Jan 28.
Polymers imprinted with sucrose and corresponding non-imprinted polymers are prepared photo-chemically at 3 degrees C and thermally at 65 degrees C. The pre-polymerization complex formation in dimethyl sulfoxide between sucrose and methacrylic acid via hydrogen bonding was investigated through (1)H NMR titration. The imprinting effect and the selectivity of the imprinted polymers in water are studied by batch rebinding studies with different mono and disaccharides and fitted to the Freundlich isotherm. Based on the calculated numbers of binding sites and average affinity, it is concluded that sucrose has been successfully imprinted at 3 and 65 degrees C. The polymer imprinted at 3 degrees C possesses the best recognition properties. The imprinted polymers are selective towards sucrose in water.
采用光化学法在 3°C 和热化学法在 65°C 条件下制备了印迹有蔗糖的聚合物和相应的非印迹聚合物。通过（1）H NMR 滴定研究了蔗糖和甲基丙烯酸在二甲基亚砜中通过氢键形成预聚合配合物的过程。通过与不同的单糖和二糖进行批量结合研究，研究了印迹聚合物在水中的印迹效应和选择性，并拟合了弗伦德利希等温线。根据计算的结合位点数量和平均亲和力，得出结论，蔗糖已在 3°C 和 65°C 下成功印迹。在 3°C 下聚合的聚合物具有最佳的识别性能。印迹聚合物对水中的蔗糖具有选择性。
