Yang Jing, Peng Bin
Department of Operative Dentistry and Endodontics, School and Hospital of Stomatology, Wuhan University, Wuhan City, China.
J Endod. 2008 Jan;34(1):22-5. doi: 10.1016/j.joen.2007.07.034. Epub 2007 Nov 26.
The experiment was undertaken to observe the correlation between the expression of c-Fos and osteoclasts in experimental periapical lesions in rats. Apical periodontitis was induced in Wistar rats by occlusal exposure of the pulp of their mandibular first molars. After the exposure, 25 Wistar rats were killed on days 0, 7, 14, 21, and 28. The jaws that contained the first molar were obtained and routinely prepared for immunohistochemistry and enzyme histochemistry. A few c-Fos-positive cells and osteoclasts could be observed on day 7, and both climaxed on day 14. In the 21-day and 28-day samples, the expression of c-Fos decreased, and fewer osteoclasts could be observed. From day 7 to day 28, a significant positive correlation was found between the expression of c-Fos and osteoclasts. These findings demonstrated that c-Fos might be associated with the differentiation of osteoclasts in the periapical lesions.
本实验旨在观察大鼠实验性根尖周病变中c-Fos表达与破骨细胞之间的相关性。通过暴露Wistar大鼠下颌第一磨牙的牙髓诱导根尖周炎。暴露后，在第0、7、14、21和28天处死25只Wistar大鼠。获取包含第一磨牙的颌骨并常规制备用于免疫组织化学和酶组织化学。在第7天可观察到一些c-Fos阳性细胞和破骨细胞，两者在第14天达到高峰。在21天和28天的样本中，c-Fos表达下降，且观察到的破骨细胞较少。从第7天到第28天，发现c-Fos表达与破骨细胞之间存在显著正相关。这些结果表明，c-Fos可能与根尖周病变中破骨细胞的分化有关。
