大豆（Glycine max (L.) Merr.）种子的酚类物质含量及抗氧化特性
Phenolic content and antioxidant properties of soybean (Glycine max (L.) Merr.) seeds.
作者信息
Malencić Djordje, Popović Milan, Miladinović Jegor
机构信息
Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia.
出版信息
Molecules. 2007 Mar 21;12(3):576-81. doi: 10.3390/12030576.
The contents and antioxidant ability of various classes of phenolic compounds present in the seeds of twenty soybean hybrids were evaluated. Total phenolics, tannins and proanthocyanidins were determined spectrophotometrically, after extraction of seeds with 70% aqueous acetone. In addition, the flavonoid contents were determined. The antioxidant activity of aqueous acetone extracts was evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay. The highest contents of total phenolics were found in Serbian cultivar 1511 and Chinese cultivar LN92-7369, which also displayed the highest total antioxidant activity. Conversely, genotypes poor in phenolics also showed low levels of DPPH-radical scavenging activity. The results suggested that besides protein and oil contents, the phenolic contents should be also considered as an important characteristic feature of soybean seeds, and as a potential selection criterion for antioxidant activity in soybean.
对20个大豆杂交种种子中各类酚类化合物的含量及抗氧化能力进行了评估。用70%的丙酮水溶液提取种子后，采用分光光度法测定总酚、单宁和原花青素的含量。此外，还测定了黄酮类化合物的含量。通过1,1-二苯基-2-苦基肼（DPPH）自由基清除活性测定法评估丙酮水溶液提取物的抗氧化活性。塞尔维亚品种1511和中国品种LN92-7369的总酚含量最高，它们也表现出最高的总抗氧化活性。相反，酚类物质含量低的基因型DPPH自由基清除活性也较低。结果表明，除蛋白质和油含量外，酚类物质含量也应被视为大豆种子的一个重要特征，以及大豆抗氧化活性的潜在选择标准。
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