Kim Hye Ryoun, Lee Mi Kyung, Park Ae Ja
Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Korea.
Korean J Lab Med. 2006 Feb;26(1):58-63. doi: 10.3343/kjlm.2006.26.1.58.
Recently, two G --> A polymorphisms at positions -308 and -238, in the promoter of the tumor necrosis factor-alpha (TNF-alpha) gene, have been identified. These variants have been linked to estimates of insulin resistance and obesity in different ethnic groups. The objective of the present study was to investigate whether these genetic variants of TNF-alpha were associated with features of the insulin resistance in two study populations comprising type 2 diabetic patients and healthy control subjects.
We analyzed the polymorphisms of TNF-alpha gene in 198 type 2 diabetes mellitus (DM) patients and 169 healthy control subjects. We used five primers and two separate polymerase chain reaction (PCR) to detect the TNF-alpha polymorphism by the multiplex amplification refractory mutation system (ARMS) technique.
No statistically significant difference in the -308A and -238A allele frequencies was found between patients with type 2 DM and normal controls.
Our study does not support a major role of the nucleotide -308 or -238 substitutions of the TNF-alpha gene in the pathogenesis of insulin resistance.
最近，已在肿瘤坏死因子-α（TNF-α）基因启动子的 -308 和 -238 位点鉴定出两个 G→A 多态性。这些变体已与不同种族群体中胰岛素抵抗和肥胖的评估相关联。本研究的目的是调查 TNF-α 的这些基因变体是否与包括 2 型糖尿病患者和健康对照受试者的两个研究人群中的胰岛素抵抗特征相关。
我们分析了 198 例 2 型糖尿病（DM）患者和 169 例健康对照受试者中 TNF-α 基因的多态性。我们使用五种引物和两次单独的聚合酶链反应（PCR），通过多重扩增不应性突变系统（ARMS）技术检测 TNF-α 多态性。
2 型糖尿病患者与正常对照之间在 -308A 和 -238A 等位基因频率上未发现统计学上的显著差异。
我们的研究不支持 TNF-α 基因的 -308 或 -238 位点核苷酸替换在胰岛素抵抗发病机制中起主要作用。
