Han Dongoh, Kim Hee-Young, Lee Hye-Jung, Shim Insop, Hahm Dae-Hyun
Acupuncture & Meridian Science Research Center, Institute of Oriental Medicine, Department of Meridian & Acupuncture,College of Oriental Medicine, Kyung-Hee University, Seoul, Korea.
J Microbiol Biotechnol. 2007 Oct;17(10):1661-9.
Gamma-aminobutyric acid (GABA) is a non-protein amino acid. It is well known for its role as an inhibitory neurotransmitter of developing and operating nervous systems in brains. In this study, a novel function of GABA in the healing process of cutaneous wounds was presented regarding anti-inflammation and fibroblast cell proliferation. The cell proliferation activity of GABA was verified through an MTT assay using murine fibroblast NIH3T3 cells. It was observed that GABA significantly inhibited the mRNA expression of iNOS, IL-1beta, and TNF-alpha, in LPS-stimulated RAW 264.7 cells. To evaluate in vivo activity of GABA in wound healing, excisional open wounds were made on the dorsal sides of Sprague-Dawley rats under anesthesia, and the healing of the wounds was apparently assessed. The molecular aspects of the healing process were also investigated by hematoxylineosin staining of the healed skin, displaying the degrees of reepithelialization and linear alignment of the granulation tissue, and immunostaining and RT-PCR analyses of fibroblast growth factor and platelet-derived growth factor, implying extracellular matrix synthesis and remodeling of the skin. The GABA treatment was effective to accelerate the healing process by suppressing inflammation and stimulating reepithelialization, compared with the epidermal growth factor treatment. The healing effect of GABA was remarkable at the early stage of wound healing, which resulted in significant reduction of the whole healing period.
γ-氨基丁酸（GABA）是一种非蛋白质氨基酸。它作为大脑中发育和运行的神经系统的抑制性神经递质而广为人知。在本研究中，提出了GABA在皮肤伤口愈合过程中的一种新功能，即抗炎和成纤维细胞增殖。通过使用小鼠成纤维细胞NIH3T3细胞的MTT试验验证了GABA的细胞增殖活性。观察到GABA显著抑制LPS刺激的RAW 264.7细胞中iNOS、IL-1β和TNF-α的mRNA表达。为了评估GABA在伤口愈合中的体内活性，在麻醉下于Sprague-Dawley大鼠背部制作切除性开放性伤口，并明显评估伤口的愈合情况。还通过对愈合皮肤进行苏木精-伊红染色来研究愈合过程的分子层面，显示再上皮化程度和肉芽组织的线性排列，并对成纤维细胞生长因子和血小板衍生生长因子进行免疫染色和RT-PCR分析，这意味着皮肤的细胞外基质合成和重塑。与表皮生长因子治疗相比，GABA治疗通过抑制炎症和刺激再上皮化有效地加速了愈合过程。GABA的愈合效果在伤口愈合早期显著，这导致整个愈合期显著缩短。
