Lee Sang Hyon, Otawa Kenichi, Onuki Motoharu, Satoh Hiroyasu, Mino Takashi
Institute of Environmental Studies, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, Japan.
J Microbiol Biotechnol. 2007 Oct;17(10):1704-7.
Monitoring of the phage-host system of Microlunatus phosphovorus indigenous in activated sludge was attempted. A laboratory-scale activated sludge process was operated for 5 weeks with synthetic wastewater. The phage-host system population in the process was monitored by plaque assay and FISH methods at every 3 days. During the process operation, the phage-host system populations were more or less steady, except for 1 week in the middle of the operation. In that period, initially M. phosphovorus decreased significantly and its lytic bacteriophages increased, and then M. phosphovorus increased back to its original level while its lytic bacteriophages decreased. This observation suggests that lytic bacteriophages should be considered as one of the biological factors affecting the bacterial population dynamics in activated sludge processes.
尝试对活性污泥中天然存在的磷微小月菌的噬菌体-宿主系统进行监测。采用合成废水在实验室规模下运行活性污泥工艺5周。每3天通过噬菌斑测定法和荧光原位杂交（FISH）方法对该工艺中的噬菌体-宿主系统种群进行监测。在工艺运行期间，噬菌体-宿主系统种群或多或少保持稳定，但在运行中期有1周除外。在那段时期，最初磷微小月菌显著减少，其裂解性噬菌体增加，然后磷微小月菌又回升至原始水平，而其裂解性噬菌体减少。这一观察结果表明，裂解性噬菌体应被视为影响活性污泥工艺中细菌种群动态的生物因素之一。
