Choi Jeongdong, Kotay Shireen Meher, Goel Ramesh
Department of Civil and Environmental Engineering; University of Utah, Salt Lake City, USA.
Bioeng Bugs. 2011 Jul-Aug;2(4):214-7. doi: 10.1016/j.watres.2010.08.038. Epub 2011 Jul 1.
In a previous paper, the first ever application of lytic bacteriophage (virus)-mediated biocontrol of biomass bulking in the activated sludge process using Haliscomenobacter hydrossis as a model filamentous bacterium was demonstrated. In this work we extended the biocontrol application to another predominant filamentous bacterium, Sphaerotilus natans, notoriously known to cause filamentous bulking in wastewater treatment systems. Very similar to previous study, one lytic bacteriophage was isolated from wastewater that could infect S. natans and cause lysis. Significant reduction in sludge volume index and turbidity of the supernatant was observed in batches containing S. natans biomass following addition of lytic phages. Microscopic examination confirmed that the isolated lytic phage can trigger the bacteriolysis of S. natans. This extended finding further strengthens our hypothesis of bacteriophage-based biocontrol of overgrowth of filamentous bacteria and the possibility of phage application in activated sludge processes, the world's widely used wastewater treatment processes.
在之前的一篇论文中，首次证明了以水生哈氏菌作为模型丝状菌，利用裂解性噬菌体（病毒）介导对活性污泥法中生物量膨胀进行生物控制。在这项工作中，我们将生物控制应用扩展到另一种主要的丝状菌——浮游球衣菌，众所周知它会在废水处理系统中导致丝状膨胀。与之前的研究非常相似，从废水中分离出一种能感染浮游球衣菌并导致其裂解的裂解性噬菌体。在添加裂解性噬菌体后，含有浮游球衣菌生物量的批次中，污泥体积指数和上清液浊度显著降低。显微镜检查证实，分离出的裂解性噬菌体可引发浮游球衣菌的细菌裂解。这一扩展发现进一步强化了我们基于噬菌体对丝状菌过度生长进行生物控制的假设，以及噬菌体应用于活性污泥法（世界广泛使用的废水处理工艺）的可能性。
