Khan M A, Satoh H, Mino T, Katayama H, Kurisu F, Matsuo T
Department of Urban and Environmental Engineering, University of Tokyo, Japan.
Water Sci Technol. 2002;46(1-2):39-43.
Bacteriophages were isolated from a laboratory scale enhanced biological phosphate removing (EBPR) activated sludge process, and their host range was examined. Bacterial isolates to host the bacteriophages were isolated from the EBPR activated sludge process. Bacteriophages were eluted from the EBPR activated sludge, enriched by incubation with the bacterial isolates, and then tested for plaque formation on each of the bacterial isolates. Out of 12 bacterial isolates isolated, 4 supported plaque formation. Four bacteriophages were obtained from the plaques. The host range test was conducted with the combination of the bacteriophage isolates and the bacterial isolates. Three of the bacteriophages were found to form plaques on more than one host, and one of them formed plaques on both gram +ve and gram -ve bacterial isolates. Two of the four bacteriophages failed to form plaques on their original bacterial host, indicating the existence of mutation on either both or one of the host and the bacteriophage. This study strongly suggests that bacteriophages are an active part of the activated sludge microbial ecosystem, having very complex interaction with their host bacteria.
从实验室规模的强化生物除磷（EBPR）活性污泥工艺中分离出噬菌体，并检测其宿主范围。用于宿主噬菌体的细菌分离株从EBPR活性污泥工艺中分离得到。噬菌体从EBPR活性污泥中洗脱，通过与细菌分离株共同培养进行富集，然后检测其在每种细菌分离株上形成噬菌斑的情况。在分离出的12株细菌分离株中，有4株支持噬菌斑形成。从这些噬菌斑中获得了4种噬菌体。对噬菌体分离株和细菌分离株的组合进行了宿主范围测试。发现其中3种噬菌体可在不止一种宿主上形成噬菌斑，其中一种在革兰氏阳性和革兰氏阴性细菌分离株上均形成噬菌斑。4种噬菌体中有2种未能在其原始细菌宿主上形成噬菌斑，这表明宿主和噬菌体两者或其中之一存在突变。这项研究有力地表明，噬菌体是活性污泥微生物生态系统的活跃组成部分，与其宿主细菌有着非常复杂的相互作用。
