聚合酶链式反应介导的质粒DNA缺失

PCR-mediated deletion of plasmid DNA.

作者信息

Hansson Mattias D, Rzeznicka Kamila, Rosenbäck Matilda, Hansson Mats, Sirijovski Nick

机构信息

Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, S-22100 Lund, Sweden.

出版信息

Anal Biochem. 2008 Apr 15;375(2):373-5. doi: 10.1016/j.ab.2007.12.005. Epub 2007 Dec 8.

DOI:10.1016/j.ab.2007.12.005

PMID:18157935

Abstract

The PCR-mediated plasmid DNA deletion method is a simple approach to delete DNA sequences from plasmids using only one round of PCR, with two primers, and without ligation or purification prior to in vivo recombination. By using only PCR, the method is sequence independent and, as shown in this study, is applicable to various sizes of plasmids and deletions using minimal primer design.

摘要

PCR介导的质粒DNA缺失方法是一种简单的方法，仅通过一轮PCR、使用两个引物，且在体内重组前无需连接或纯化，就能从质粒中删除DNA序列。仅通过PCR，该方法不依赖序列，并且如本研究所示，使用最少的引物设计即可适用于各种大小的质粒和缺失操作。