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大麦微管蛋白基因的克隆与表达

Cloning and expression of the tubulin genes in barley.

作者信息

Radchuk Volodymyr, Sreenivasulu Nese, Blume Yaroslav, Weschke Winfriede

机构信息

Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, D-06466 Gatersleben, Germany.

出版信息

Cell Biol Int. 2008 May;32(5):557-9. doi: 10.1016/j.cellbi.2007.11.009. Epub 2007 Nov 21.

DOI:10.1016/j.cellbi.2007.11.009
PMID:18158258
Abstract

Tubulins are encoded by small gene families in plants. Based on the barley EST collection, cDNAs for alpha-, beta-, and gamma-tubulins were selected. Five genes for alpha-tubulin, eight newly identified beta-tubulin sequences and one gamma-tubulin gene were found to be expressed in barley. In silico analysis of relative abundance of the distinct tubulin sequences among ESTs derived from different libraries revealed that the various tubulin genes differed in their level of expression, and to some extent were tissue specific.

摘要

微管蛋白由植物中的小基因家族编码。基于大麦EST文库,挑选出了α-、β-和γ-微管蛋白的cDNA。发现大麦中有5个α-微管蛋白基因、8个新鉴定的β-微管蛋白序列和1个γ-微管蛋白基因表达。对来自不同文库的EST中不同微管蛋白序列的相对丰度进行电子分析表明,各种微管蛋白基因的表达水平不同,且在一定程度上具有组织特异性。

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Cloning and expression of the tubulin genes in barley.大麦微管蛋白基因的克隆与表达
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引用本文的文献

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Alpha-tubulin (CsTUA) up-regulated during winter dormancy is a low temperature inducible gene in tea [Camellia sinensis (L.) O. Kuntze].在冬季休眠期间上调的α-微管蛋白(CsTUA)是茶树(Camellia sinensis(L.)O. Kuntze)中低温诱导的基因。
Mol Biol Rep. 2012 Apr;39(4):3485-90. doi: 10.1007/s11033-011-1121-7. Epub 2011 Jul 3.