Viegas Vinicius Nery, Abreu Marcelo Emir Requia, Viezzer Christian, Machado Denise Cantarelli, Filho Manoel Sant'anna, Silva Daniela Nascimento, Pagnoncelli Rogério Miranda
School of Dentistry, Laser Center, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
Photomed Laser Surg. 2007 Dec;25(6):467-73. doi: 10.1089/pho.2007.1098.
This study evaluated the action of low-level laser therapy (LLLT) on the modulation of inflammatory reactions during wound healing in comparison with meloxicam.
LLLT has been recommended for the postoperative period because of its ability to speed healing of wounds. However, data in the literature are in disagreement about its anti-inflammatory action.
Standardized circular wounds were made on the backs of 64 Wistar rats. The animals were divided into four groups according to the selected postoperative therapy: group A-control; group B-administration of meloxicam; and groups C and D-irradiation with red (lambda = 685 nm) and infrared (lambda = 830 nm) laser energy, respectively. The animals were killed at 12, 36, and 72 h and 7 days after the procedure.
Microscopic analysis revealed significant vascular activation of irradiated sites in the first 36 h. Only group B showed decreases in the intensity of polymorphonuclear infiltrates and edema. Group D showed a higher degree of organization and maturation of collagen fibers than the other groups at 72 h. The animals in group C showed the best healing pattern at 7 days. The anti-inflammatory action of meloxicam was confirmed by the results obtained in this research. The quantification of interleukin-1beta (IL-1beta) mRNA by real-time polymerase chain reaction (PCR) did not show any reduction in the inflammatory process in the irradiated groups when compared to the other groups.
LLLT improves the quality of histologic repair and is useful during wound healing. However, with the methods used in this study the laser energy did not minimize tissue inflammatory reactions.
本研究评估低强度激光疗法（LLLT）与美洛昔康相比，在伤口愈合过程中对炎症反应调节的作用。
由于LLLT具有加速伤口愈合的能力，已被推荐用于术后阶段。然而，文献中的数据对于其抗炎作用存在分歧。
在64只Wistar大鼠的背部制作标准化圆形伤口。根据所选的术后治疗方法，将动物分为四组：A组为对照组；B组给予美洛昔康；C组和D组分别用红色（λ = 685 nm）和红外（λ = 830 nm）激光能量照射。在手术后12、36、72小时和7天处死动物。
显微镜分析显示，在最初的36小时内，照射部位有明显的血管激活。只有B组的多形核浸润强度和水肿程度有所降低。在72小时时，D组的胶原纤维组织化和成熟程度高于其他组。C组的动物在7天时显示出最佳的愈合模式。本研究结果证实了美洛昔康的抗炎作用。与其他组相比，通过实时聚合酶链反应（PCR）对白细胞介素-1β（IL-1β）mRNA进行定量分析，未显示照射组炎症过程有任何减轻。
LLLT可提高组织学修复质量，在伤口愈合过程中有用。然而，采用本研究中的方法，激光能量并未使组织炎症反应降至最低。
