Beigom Taheri Jamileh, Bagheri Fatemeh, Mojahedi Masood, Shamloo Nafise, Nakhostin Mohammad Reza, Azimi Somayyeh, Namazi Zahra
School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2015 Summer;6(3):124-8. doi: 10.15171/jlms.2015.06. Epub 2015 Jun 28.
Different modalities have been used to achieve better results in wound healing among which are laser and pharmacological agents. To our knowledge, there is no investigation to compare phenytoin and laser together. In this study, we aimed to evaluate laser and phenytoin efficacy and compare their effect on wound healing.
We used 60 rats. The animals were divided into 4 groups, 15 rats in each and each group was subdivided into 3 subgroups for which postoperative evaluation was performed on days 3, 7, and 14. After general anesthesia and back hair shaving of each rat, an excisional wound was made. The wounds in group 2 were treated with 1% phenytoin cream. In group 3, the wounds were irradiated with diode laser. The wounds of group 4 were treated with both phenytoin cream and laser administration. The animals of group 1 served as control. For histopathological evaluation, hematoxylin and eosin staining was used. Polymorphonuclear leucocytes (PMNs), re-epithelization, fibroblasts, new vessels and collagen were evaluated.
Faster reduction in polymorphonuclear cells after 7 and 14 days and more collagen fibers on the third day in laser treated group was statistically significant (P < .05) .The result for the phenytoin treated group were not better than control group. When both laser and phenytoin were used samples showed more collagen fibers on the third day, which was statistically significant (P < .05).
It seems that low level laser is effective in skin wound healing and have better results than phenytoin application.
为了在伤口愈合方面取得更好的效果，人们使用了不同的方法，其中包括激光和药物制剂。据我们所知，尚无研究将苯妥英钠和激光一起进行比较。在本研究中，我们旨在评估激光和苯妥英钠的疗效，并比较它们对伤口愈合的影响。
我们使用了60只大鼠。将动物分为4组，每组15只，每组又细分为3个亚组，分别在术后第3天、第7天和第14天进行评估。在对每只大鼠进行全身麻醉并剃去背部毛发后，制作一个切除性伤口。第2组的伤口用1%苯妥英钠乳膏治疗。第3组的伤口用二极管激光照射。第4组的伤口同时用苯妥英钠乳膏和激光治疗。第1组的动物作为对照。用于组织病理学评估的是苏木精和伊红染色。评估了多形核白细胞（PMN）、再上皮化、成纤维细胞、新血管和胶原蛋白。
激光治疗组在第7天和第14天后多形核细胞减少更快，且在第3天有更多的胶原纤维，差异有统计学意义（P < 0.05）。苯妥英钠治疗组的结果并不优于对照组。当同时使用激光和苯妥英钠时，样本在第3天显示出更多的胶原纤维，差异有统计学意义（P < 0.05）。
低强度激光似乎对皮肤伤口愈合有效，且比应用苯妥英钠有更好的效果。
