Yang Zhengpeng, Si Shihui, Zhang Chunjing
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Biochem Biophys Res Commun. 2008 Feb 29;367(1):169-75. doi: 10.1016/j.bbrc.2007.12.113. Epub 2007 Dec 26.
Magnetic single-enzyme nanoparticles (SENs) encapsulated within a composite inorganic/organic polymer network were fabricated via the surface modification and in situ aqueous polymerization of separate enzyme molecule. The resultant nanoparticles were characterized by transmission electron microscope (TEM), Fourier transform infrared (FTIR) spectrometer and X-ray diffraction (XRD). These particles are almost spherical in shape and have a unique size of about 50 nm in diameter. Electrical and magnetic measurements reveal that the magnetic SENs have a conductivity of 2.7 x 10(-3)Scm(-1), and are superparamagnetic with a saturation magnetization of 14.5e microg(-1) and a coercive force of 60Oe. Compared with free enzyme, encapsulated enzyme exhibits a strong tolerance to the variation of solution pH, high temperature, organic solvent and long-term storage, thus showing significantly enhanced enzyme performance and stability.
通过对单个酶分子进行表面修饰和原位水相聚合，制备了包裹在无机/有机复合聚合物网络中的磁性单酶纳米颗粒（SENs）。通过透射电子显微镜（TEM）、傅里叶变换红外（FTIR）光谱仪和X射线衍射（XRD）对所得纳米颗粒进行了表征。这些颗粒形状近似球形，直径约为50 nm，具有独特的尺寸。电学和磁学测量表明，磁性SENs的电导率为2.7×10⁻³ S cm⁻¹，具有超顺磁性，饱和磁化强度为14.5 emu g⁻¹，矫顽力为60 Oe。与游离酶相比，包封酶对溶液pH值变化、高温、有机溶剂和长期储存具有较强的耐受性，从而显著提高了酶的性能和稳定性。
