Bio-Nano Electronics Research Centre, Toyo University, Saitama, Japan.
PLoS One. 2013 Jun 14;8(6):e66528. doi: 10.1371/journal.pone.0066528. Print 2013.
We immobilize hydrolases such as lipase and chitinase on superparamagnetic particles, which are subjected to a rotational magnetic field, and measure the activities of the enzymes. We find that the activities of lipase and chitinase increase in the rotational magnetic field compared to those in the absence of a magnetic field and reach maximum at certain frequencies. The present methodology may well be utilized for the design and development of efficient micro reactors and micro total analysis systems (μ-TASs).
我们将脂肪酶和几丁质酶等水解酶固定在超顺磁颗粒上，然后将其置于旋转磁场中，并测量酶的活性。我们发现，与没有磁场的情况相比，脂肪酶和几丁质酶在旋转磁场中的活性增加，并在某些频率下达到最大值。本方法有望用于设计和开发高效的微反应器和微全分析系统（μ-TASs）。
