[醛固酮对豚鼠耳蜗水通道蛋白和离子载体蛋白表达的影响]
[Effect of aldosterone on aquaporin and ionophorous protein expressions in guinea pig cochlea].
作者信息
Li Qi, Huang De-Liang
机构信息
Department of Otolaryngology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2007 Dec;27(12):1918-20.
OBJECTIVE
To study the effect of aldosterone on aquaporin and ionophorous protein expressions in the cochlea of guinea pigs.
METHODS
RT-PCR was used to detect the expression of Na-K ATPase and epithelial sodium channel (ENaC) mRNA in the cochlea of guinea pigs 6 h after intraperitoneal aldosterone injection. Immunohistochemistry was used to detect the expression of aquaporin 1(AQP1) in the cochlea 1 month after the injection.
RESULTS
Early after aldosterone injection, the expression level of Na-K ATPase beta(1) and beta(3) subunit mRNAs remained unchanged and level of ENaC alpha subunit mRNA was up-regulated in the cochlear lateral wall (P<0.05), and 1 month after the injection, the expression of AQP1 protein was down-regulated (P<0.05).
CONCLUSION
The effect of aldosterone on the cochlea is mediated possibly through its action on the genome, and aldosterone may cause ion concentration alterations to induce endolymphatic hydrops.
目的
研究醛固酮对豚鼠耳蜗水通道蛋白及离子载体蛋白表达的影响。
方法
采用逆转录聚合酶链反应（RT-PCR）检测腹腔注射醛固酮6小时后豚鼠耳蜗中钠钾ATP酶（Na-K ATPase）及上皮钠通道（ENaC）mRNA的表达。采用免疫组织化学法检测注射后1个月耳蜗中 aquaporin 1（AQP1）的表达。
结果
醛固酮注射后早期，耳蜗外侧壁中Na-K ATPase β(1)和β(3)亚基mRNA的表达水平保持不变，而ENaC α亚基mRNA的水平上调（P<0.05），注射后1个月，AQP1蛋白的表达下调（P<0.05）。
结论
醛固酮对耳蜗的作用可能是通过其对基因组的作用介导的，醛固酮可能导致离子浓度改变，从而诱发内淋巴积水。
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