Ding Tianbing, Song Haengseok, Wang Xiaohong, Khatua Atanu, Paria Bibhash C
Division of Neonatology, Department of Pediatrics, Vanderbilt University Medical Center, 1125 MRB IV/Light Hall, 2215 B. Garland Avenue, Nashville, TN 37232-0656, USA.
Reproduction. 2008 Jan;135(1):41-53. doi: 10.1530/REP-07-0013.
Blastocyst implantation occurs in the progesterone-primed uterus of hamsters, but not in mice where the progesterone-primed uterus requires estrogen influence. Leukemia inhibitory factor (Lif), an estrogen-regulated gene in mice, is an absolutely needed cytokine for uterine receptivity and implantation in this species. This study aimed to evaluate the importance of Lif ligand-receptor signaling during uterine receptivity and implantation in hamsters. We investigated whether or not the uterine expression patterns of Lif and its receptors, Lif-r and gp130, during the periimplantation period of pregnancy and its hormonal regulation in the ovariectomized hamster correlate with some of the vital phases of uterine changes during early pregnancy. Uterine Lif, Lif-r, and gp130 mRNA expressions were examined by Northern and in situ hybridization. During the uterine preparatory phase for implantation, Lif, Lif-r, and gp130 were expressed either in the gland, luminal epithelium or both. As the implantation process began, Lif expression was minimal, but Lif-r and gp130 extended to the decidual areas. This decidual expression of Lif-r and gp130 was not dependent on the presence of the embryo since these genes were expressed in the suture-induced deciduomata. We also observed that, while the uterine Lif was induced by estrogen, Lif-r and gp130 were induced by progesterone in ovariectomized hamsters. Additionally, we show that a Lif antibody when instilled intraluminally on day 3 of pregnancy reduced the number of implantation sites. Taken together, these data suggest that Lif signaling is important for uterine receptivity and implantation in hamsters.
囊胚着床发生在经孕酮预处理的仓鼠子宫中，但在小鼠中不会发生，在小鼠中经孕酮预处理的子宫需要雌激素的影响。白血病抑制因子（Lif）是小鼠中的一种雌激素调节基因，是该物种子宫接受性和着床绝对必需的细胞因子。本研究旨在评估Lif配体 - 受体信号在仓鼠子宫接受性和着床过程中的重要性。我们研究了妊娠植入前期Lif及其受体Lif-r和gp130在子宫中的表达模式，以及在去卵巢仓鼠中其激素调节是否与妊娠早期子宫变化的一些关键阶段相关。通过Northern杂交和原位杂交检测子宫Lif、Lif-r和gp130 mRNA的表达。在子宫植入准备阶段，Lif、Lif-r和gp130在腺体、腔上皮或两者中均有表达。随着植入过程开始，Lif表达降至最低，但Lif-r和gp130扩展到蜕膜区域。Lif-r和gp130在蜕膜的这种表达不依赖于胚胎的存在，因为这些基因在缝线诱导的蜕膜瘤中表达。我们还观察到，虽然子宫Lif由雌激素诱导，但在去卵巢仓鼠中，Lif-r和gp130由孕酮诱导。此外，我们发现，在妊娠第3天经宫腔内注入Lif抗体可减少着床点的数量。综上所述，这些数据表明Lif信号对于仓鼠子宫接受性和着床很重要。
