Yoon Ie-na, Lee Dong-hoon, Rah Sang-hoon
Department of Ophthalmology, Wonju Christian Hospital, Yonsei University, Wonju College of Medicine, 162 Ilsan-dong, Wonju 220-701, Korea.
Yonsei Med J. 2007 Dec 31;48(6):955-62. doi: 10.3349/ymj.2007.48.6.955.
In the field of ophthalmology, the conjunctival autograft is a useful therapeutic material in many cases, but the small size of the autograft is a disadvantage. Therefore, we evaluated the feasibility of taking an expanded sample of conjunctival tissue using a subtenon's silicone implant.
We included a total of nine rabbits; eight rabbits were operative cases, and one was a control. A portion of conjunctival tissue from the control rabbit, which did not undergo surgery, was dissected and examined to determine whether it was histologically different from the experimental group. The surgical procedure was performed on eight rabbits via a subtenon's insertion of a silicone sponge in the left superior-temporal portion; after surgery, we dropped antibiotics into the eyes. We sacrificed a pair of rabbits every three days (on days 3, 6, 9, and 12) after surgery, removed the expanded conjunctival tissues with the silicone sponge implants, and measured their sizes.
The mean size of the expanded conjunctival tissues was 194.4 mm2. On the third day, we were able to harvest a 223.56 mm2 section of conjunctival tissue, which was the most expanded sample of tissue in the study. On the twelfth day, we removed a 160.38 mm2 section of conjunctival tissue, which was the least expanded sample of tissue. Statistically, there were no significant differences in the mean dimensions of the expanded conjunctival tissues for each time period. Microscopic examinations showed no histological differences between the expanded conjunctival tissues and the normal conjunctival tissues.
The results reveal that this procedure is a useful method to expand the conjunctiva for grafting and transplantation.
在眼科领域，结膜自体移植在许多情况下是一种有用的治疗材料，但自体移植片尺寸较小是一个缺点。因此，我们评估了使用颞下硅胶植入物获取扩大的结膜组织样本的可行性。
我们共纳入9只兔子；8只为手术病例，1只为对照。解剖未接受手术的对照兔的一部分结膜组织并进行检查，以确定其在组织学上是否与实验组不同。通过在8只兔子的左上颞部经颞下插入硅胶海绵进行手术；术后，我们向眼中滴入抗生素。术后每三天（第3、6、9和12天）处死一对兔子，取出带有硅胶海绵植入物的扩大结膜组织，并测量其大小。
扩大结膜组织的平均大小为194.4平方毫米。在第三天，我们能够获取一片223.56平方毫米的结膜组织切片，这是该研究中组织扩张程度最大的样本。在第十二天，我们取出一片160.38平方毫米的结膜组织切片，这是组织扩张程度最小的样本。统计学上，每个时间段扩大结膜组织的平均尺寸没有显著差异。显微镜检查显示，扩大结膜组织与正常结膜组织之间在组织学上没有差异。
结果表明，该方法是一种用于扩大结膜以进行移植的有用方法。
