Abdelwahed Afef, Skandrani Ines, Kilani Soumaya, Neffati A, Sghaier Mohamed Ben, Bouhlel Ines, Boubaker Jihed, Ammar Rebaï Ben, Mahmoud Amor, Ghedira Kamel, Chekir-Ghedira Leila
Unité de Pharmacognosie/Biologie Moléculaire, Faculté de Pharmacie de Monastir, Monastir, Tunisie.
Drug Chem Toxicol. 2008;31(1):37-60. doi: 10.1080/01480540701688634.
Mutagenic and antimutagenic activities against direct acting mutagens, nifuroxazide (NF) and sodium azide (SA), and indirect acting mutagen aflatoxin B1 (AFB1) of extracts prepared from aerial parts of Pituranthos tortuosus were investigated in bacterial assay systems (i.e., the Ames test with Salmonella typhimurium TA100, TA98, TA1538, TA1535, and the SOS chromotest with Escherichia coli PQ 37). It was found that all extracts obtained from P. tortuosus decreased the mutagenicity induced by AFB1 (10 microg/assay), SA (1.5 microg/assay), and NF (20 microg/assay). Ethyl acetate, acetone, methanol, and total oligomer flavenoid extracts exhibited the highest inhibition level of mutagenicity induced by the indirect mutagen AFB1. In addition, antiproliferative and apoptotic properties of these extracts have also been reported using two leukemia cell lines, L1210 and K562. The results revealed that all extracts showed a significant cytotoxic effect on these cell lines, and the effect was greater in the presence of human K562 chronic myelogenous leukemia cells, whereas they do not induce apoptosis.
在细菌检测系统（即鼠伤寒沙门氏菌TA100、TA98、TA1538、TA1535的艾姆斯试验以及大肠杆菌PQ 37的SOS显色试验）中，研究了从扭曲叶苞菊地上部分制备的提取物对直接作用诱变剂硝呋柳氮（NF）和叠氮化钠（SA）以及间接作用诱变剂黄曲霉毒素B1（AFB1）的诱变和抗诱变活性。结果发现，从扭曲叶苞菊获得的所有提取物均降低了由AFB1（10微克/试验）、SA（1.5微克/试验）和NF（20微克/试验）诱导的诱变性。乙酸乙酯、丙酮、甲醇和总低聚黄酮提取物对间接诱变剂AFB1诱导的诱变性表现出最高的抑制水平。此外，还报道了使用两种白血病细胞系L1210和K562对这些提取物的抗增殖和凋亡特性。结果显示，所有提取物对这些细胞系均表现出显著的细胞毒性作用，在人K562慢性粒细胞白血病细胞存在的情况下作用更强，然而它们并不诱导凋亡。
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