来自日本杏（“梅”）的新型抗增殖剂MK615在体外诱导结肠癌细胞发生显著自噬。
New anti-proliferative agent, MK615, from Japanese apricot "Prunus mume" induces striking autophagy in colon cancer cells in vitro.
作者信息
Mori Shozo, Sawada Tokihiko, Okada Toshie, Ohsawa Tatsushi, Adachi Masakazu, Keiichi Kubota
机构信息
Second Department of Surgery, Dokkyo University School of Medicine, Kitakobayashi 880, Mibu, Shimotsuga, Tochigi 321-0293, Japan.
出版信息
World J Gastroenterol. 2007 Dec 28;13(48):6512-7. doi: 10.3748/wjg.v13.i48.6512.
AIM
To investigate the anti-neoplastic effects of MK615, an extract from the Japanese apricot (Prunus mume), against colon cancer cells.
METHODS
Three colon cancer cell lines, SW480, COLO, and WiDr, were cultured with MK615. Growth inhibition was evaluated by cell proliferation assay and killing activity was determined by lactate dehydrogenase assay. Induction of apoptosis was evaluated by annexin V flow cytometry. Morphological changes were studied by light and electron microscopy, and immunofluorescence staining with Atg8.
RESULTS
MK615 inhibited growth and lysed SW480, COLO and WiDr cells in a dose-dependent manner. Annexin V flow cytometry showed that MK615 induced apoptosis after 6 h incubation, at which point the occurrence of apoptotic cells was 68.0%, 65.7% and 64.7% for SW480, COLO, and WiDr cells, respectively. Light and electron microscopy, and immunofluorescence staining with Atg8 revealed that MK615 induced massive cytoplasmic vacuoles (autophagosomes) in all three cell lines.
CONCLUSION
MK615 has an anti-neoplastic effect against colon cancer cells. The effect may be exerted by induction of apoptosis and autophagy.
目的
研究青梅提取物MK615对结肠癌细胞的抗肿瘤作用。
方法
用MK615培养三种结肠癌细胞系SW480、COLO和WiDr。通过细胞增殖试验评估生长抑制情况，通过乳酸脱氢酶试验测定杀伤活性。用膜联蛋白V流式细胞术评估细胞凋亡的诱导情况。通过光学显微镜和电子显微镜研究形态学变化，并用Atg8进行免疫荧光染色。
结果
MK615以剂量依赖的方式抑制SW480、COLO和WiDr细胞的生长并使其裂解。膜联蛋白V流式细胞术显示，孵育6小时后MK615诱导细胞凋亡，此时SW480、COLO和WiDr细胞的凋亡细胞发生率分别为68.0%、65.7%和64.7%。光学显微镜和电子显微镜以及用Atg8进行的免疫荧光染色显示，MK615在所有三种细胞系中均诱导大量细胞质空泡（自噬体）形成。
结论
MK615对结肠癌细胞具有抗肿瘤作用。这种作用可能是通过诱导细胞凋亡和自噬来实现的。
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