Elema J D, Hoyer J R, Vernier R L
Kidney Int. 1976 May;9(5):395-406. doi: 10.1038/ki.1976.49.
Collodial carbon, 70 mg/100 g, was injected into rats which were sacificed for histologic study of the kidneys at intervals of five minutes to seven weeks. Transient thrombocytopenia and albuminuria were observed. Uptake of carbon by the mesangium of glomeruli was maximal at 32 hr and gradually decreased thereafter. Semiquantitative analysis of the distribution of carbon particles within glomeruli revealed a predominately peripheral localization during early time periods, and increased relative concentration of particles within more central zones and in the lacis area at the vascular pole of the glomerulus at two to seven weeks, indicating that one of the mechanisms for clearance of materials from the mesangium was movement of particles in the direction of the lacis area. Sequential electron microscopic studies showed that carbon particles moved through fenestrae in the endothelium covering the mesangium into channels between mesangial cells. Observations at later intervals suggested that carbon eventually reached the vascular pole by being passed on from one mesangial cell to the next. Vasoactive amines or other substances derived from platelets may play an important role in initiating the process of mesangial uptake.
将70毫克/100克的胶态碳注射到大鼠体内，每隔5分钟至7周处死一批大鼠，用于对肾脏进行组织学研究。观察到短暂性血小板减少和蛋白尿。肾小球系膜对碳的摄取在32小时时达到最大，此后逐渐下降。对肾小球内碳颗粒分布的半定量分析显示，在早期，碳颗粒主要位于外周，而在2至7周时，肾小球血管极处的中央区域和球旁系膜区（lacis area）内颗粒的相对浓度增加，这表明从系膜清除物质的机制之一是颗粒向球旁系膜区移动。连续电子显微镜研究表明，碳颗粒通过覆盖系膜的内皮细胞窗孔进入系膜细胞之间的通道。后期观察表明，碳最终通过从一个系膜细胞传递到下一个系膜细胞而到达血管极。血管活性胺或源自血小板的其他物质可能在启动系膜摄取过程中起重要作用。
