Ogunro P S, Ogungbamigbe T O, Elemie P O, Egbewale B E, Adewole T A
Department of Chemical Pathology, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Nigeria.
Niger Postgrad Med J. 2006 Mar;13(1):1-5.
To find a correlation, if any, between the concentration of plasma selenium and erythrocyte activity in HIV-infected patients with the progression of the disease.
Plasma selenium concentration and erythrocyte glutathione peroxidase (GSH-Px) activity were measured in 62 HIV-1 seropositive patients before commencing antiretroviral drug. Thirty (30) age-matched and apparently healthy HIV-1/11 seronegative subjects were recruited as control subjects. HIV-1 seropositive patients were classified according to the Centre for Disease Control and Prevention (CDC) Criteria.
The mean plasma selenium concentrations of 0.53+/-0.06_mol/L and 0.71+/-0.10_mol/L were significantly reduced (P<0.001) in HIV- 1 patients with CD4+ count of <200 lym/mm3 and that of 200-499 lym/mm3 respectively when compared with the controls which is 1.01+/-0.10_mol/L. The mean erythrocyte GSH-Px activity of 15.1+/-2.4 U/g Hb and 20.7+/-3.7U/g Hb of HIV-1 seropositive patients with CD4+ count of <200 lym/mm3 and 200-499 lym/mm3 respectively were significantly reduced (P<0.001) compared with 31.5+/-4.5 U/g Hb for the control. Our results clearly show that there is reduced scavenging antioxidant i.e Selenium and GSH-Px which will lead to oxidative stress in the HIV-1 patients when compared with controls, and decrease significantly with the progression of disease, i.e. AIDS<symptomatics<asymptomatics<controls. HIV-1 subtype variants have no significant influence in both parameters.
The inclusion of selenium micronutrient which is essential for the activity of erythrocyte GSH-Px as an adjuvant in the management of HIV seropositive patients to prevent additional damage that can be caused by free radicals will be of immense benefit.
探究在感染HIV的患者中，血浆硒浓度与红细胞活性之间是否存在关联，以及这种关联与疾病进展的关系。
在62例开始接受抗逆转录病毒药物治疗前的HIV-1血清阳性患者中，测量血浆硒浓度和红细胞谷胱甘肽过氧化物酶（GSH-Px）活性。招募30名年龄匹配且表面健康的HIV-1/11血清阴性受试者作为对照。HIV-1血清阳性患者根据疾病控制与预防中心（CDC）标准进行分类。
与对照组（1.01±0.10 μmol/L）相比，CD4+细胞计数<200个/μL和200-499个/μL的HIV-1患者的平均血浆硒浓度分别显著降低至0.53±0.06 μmol/L和0.71±0.10 μmol/L（P<0.001）。CD4+细胞计数<200个/μL和200-499个/μL的HIV-1血清阳性患者的平均红细胞GSH-Px活性分别为15.1±2.4 U/g Hb和20.7±3.7 U/g Hb，与对照组的31.5±4.5 U/g Hb相比显著降低（P<0.001）。我们的结果清楚地表明，与对照组相比，HIV-1患者清除抗氧化剂（即硒和GSH-Px）的能力降低，这将导致氧化应激，并且随着疾病进展（即艾滋病<有症状者<无症状者<对照组）显著下降。HIV-1亚型变体对这两个参数均无显著影响。
将对红细胞GSH-Px活性至关重要的硒微量营养素作为辅助手段纳入HIV血清阳性患者的治疗中，以防止自由基可能造成的额外损害，将具有巨大益处。
