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对从中国双峰驼(骆驼属双峰驼)分离出的安氏隐孢子虫(顶复门)进行多位点系统发育分析。

Multilocus phylogenetic analysis of Cryptosporidium andersoni (Apicomplexa) isolated from a bactrian camel (Camelus bactrianus) in China.

作者信息

Wang Rongjun, Zhang Longxian, Ning Changshen, Feng Yaoyu, Jian Fuchun, Xiao Lihua, Lu Biao, Ai Weichang, Dong Heping

机构信息

The College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, the People's Republic of China.

出版信息

Parasitol Res. 2008 Apr;102(5):915-20. doi: 10.1007/s00436-007-0851-x. Epub 2007 Dec 30.

Abstract

This is the first report of cryptosporidiosis in a bactrian camel (Camelus bactrianus) in China. Two Cryptosporidium isolates derived from the same bactrian camel (3-year-old) in November 2005 and April 2006 were characterized using sequence and phylogenetic analysis of the small-subunit rRNA (18S rRNA), 70-kDa heat shock protein (HSP70), actin and Cryptosporidium oocyst wall protein (COWP) genes. The sequences of the 18S rRNA and COWP were identical to all other Cryptosporidium andersoni isolates although minor differences were noticed between the isolates and the USA isolate at the actin locus (99.2% of similarity). The sequence of the HSP70 was identical to the Japanese C. andersoni isolate, with a minor difference from the Australian C. andersoni isolate (99.7% of similarity). Cross-transmission studies demonstrated that the C. andersoni isolates did not infect immunosuppressed or immunocompetent Kun-ming mice, severe combined immunodeficiency mice, and immunosuppressed or immunocompetent calves. Among the C. andersoni isolates reported so far, only isolates from Japan could infect SCID mice. Thus, the C. andersoni isolates from the bactrian camel were biologically similar to most bovine C. andersoni isolates characterized so far, but are different from bovine isolates from Japan.

摘要

这是中国双峰驼隐孢子虫病的首例报告。对2005年11月和2006年4月从同一峰3岁双峰驼分离得到的两株隐孢子虫,采用小亚基核糖体RNA(18S rRNA)、70 kDa热休克蛋白(HSP70)、肌动蛋白和隐孢子虫卵囊壁蛋白(COWP)基因的序列及系统发育分析进行特征鉴定。18S rRNA和COWP的序列与所有其他安氏隐孢子虫分离株相同,不过在肌动蛋白基因座上,这些分离株与美国分离株存在细微差异(相似度为99.2%)。HSP70的序列与日本安氏隐孢子虫分离株相同,与澳大利亚安氏隐孢子虫分离株存在细微差异(相似度为99.7%)。交叉传播研究表明,这些安氏隐孢子虫分离株不会感染免疫抑制或免疫健全的昆明小鼠、严重联合免疫缺陷小鼠以及免疫抑制或免疫健全的犊牛。在目前已报道的安氏隐孢子虫分离株中,只有来自日本的分离株能够感染重症联合免疫缺陷(SCID)小鼠。因此,来自双峰驼的安氏隐孢子虫分离株在生物学特性上与目前已鉴定的大多数牛源安氏隐孢子虫分离株相似,但与来自日本的牛源分离株不同。

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