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来自不同宿主的微小隐孢子虫的分子和系统发育分析。

Molecular and phylogenetic analysis of Cryptosporidium muris from various hosts.

作者信息

Morgan U M, Xiao L, Monis P, Sulaiman I, Pavlasek I, Blagburn B, Olson M, Upton S J, Khramtsov N V, Lal A, Elliot A, Thompson R C

机构信息

World Health Organization Collaborating Centre for the Molecular Epidemiology of Parasitic Infections, Murdoch, WA, Australia.

出版信息

Parasitology. 2000 May;120 ( Pt 5):457-64. doi: 10.1017/s0031182099005703.

DOI:10.1017/s0031182099005703
PMID:10840975
Abstract

Isolates of Cryptosporidium muris and C. serpentis were characterized from different hosts using nucleotide sequence analysis of the rDNA 18S and ITS1 regions, and the heat-shock (HSP-70) gene. Phylogenetic analysis confirmed preliminary evidence that C. muris is not a uniform species. Two distinct genotypes were identified within C. muris; (1) C. muris genotype A; comprising bovine and camel isolates of C. muris from different geographical locations, and (2) C. muris genotype B comprising C. muris isolates from mice, a hamster, a rock hyrax and a camel from the same enclosure. These 2 genotypes may represent separate species but further biological and molecular studies are required for confirmation.

摘要

利用核糖体DNA(rDNA)18S和ITS1区域以及热休克(HSP - 70)基因的核苷酸序列分析,对来自不同宿主的微小隐孢子虫和蛇形隐孢子虫分离株进行了特征鉴定。系统发育分析证实了初步证据,即微小隐孢子虫不是一个统一的物种。在微小隐孢子虫中鉴定出两种不同的基因型;(1)微小隐孢子虫基因型A,包括来自不同地理位置的牛和骆驼的微小隐孢子虫分离株,以及(2)微小隐孢子虫基因型B,包括来自同一围栏内小鼠、仓鼠、岩蹄兔和骆驼的微小隐孢子虫分离株。这两种基因型可能代表不同的物种,但需要进一步的生物学和分子研究来证实。

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