Baroudi Djamel, Zhang Hongwei, Amer Said, Khelef Djamel, Roellig Dawn M, Wang Yuanfei, Feng Yaoyu, Xiao Lihua
École Nationale Supérieure Vétérinaire, Rue Issad Abbes, El Alia, Alger, Algeria.
Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA, 30329, USA.
Parasitol Res. 2018 Mar;117(3):905-910. doi: 10.1007/s00436-017-5734-1. Epub 2018 Jan 6.
Little information is available on the occurrence of the zoonotic protists Cryptosporidium spp. and none on Enterocytozoon bieneusi in camels. This preliminary study was conducted to examine the identity of Cryptosporidium subtypes and E. bieneusi genotypes in dromedary camels in Algeria. A total of 39 fecal specimens were collected from young camels. PCR-sequence analysis of the small subunit rRNA was used to detect and genotype Cryptosporidium spp. Cryptosporidium parvum present was further subtyped by sequence analysis of the 60 kDa glycoprotein gene. PCR-sequence analysis of the ribosomal internal transcribed spacer gene was used to detect and genotype E. bieneusi. Altogether, two and eight of the specimens analyzed were positive for C. parvum and E. bieneusi, respectively. The former was identified as a new subtype that is genetically related to the C. hominis If subtype family, whereas the latter was identified as two related genotypes (Macaque1 and a novel genotype) in the newly assigned E. bieneusi genotype group 8. Although they are not known hosts for C. parvum and E. bieneusi, camels are apparently infected with genetically distinct variants of these pathogens.
关于人畜共患原生生物隐孢子虫属的发生情况，目前可用信息很少，而关于骆驼体内的微小隐孢子虫则毫无相关信息。本初步研究旨在检测阿尔及利亚单峰驼体内隐孢子虫亚型和微小隐孢子虫基因型的特征。从幼驼身上总共采集了39份粪便样本。利用小亚基核糖体RNA的PCR序列分析来检测隐孢子虫属并进行基因分型。对存在的微小隐孢子虫通过60 kDa糖蛋白基因的序列分析进一步进行亚型分型。利用核糖体内部转录间隔区基因的PCR序列分析来检测微小隐孢子虫并进行基因分型。总共，分别有两份和八份分析样本对微小隐孢子虫和微小隐孢子虫呈阳性。前者被鉴定为一种新的亚型，在基因上与人类隐孢子虫If亚型家族相关，而后者在新划分的微小隐孢子虫基因型8组中被鉴定为两种相关基因型（猕猴1型和一种新基因型）。尽管骆驼并非已知的微小隐孢子虫和微小隐孢子虫宿主，但显然感染了这些病原体的基因不同的变体。
