骨质疏松大鼠唾液与血清骨转换标志物之间的相关性

Correlation between salivary and serum markers of bone turnover in osteopenic rats.

作者信息

Pellegrini Gretel G, Gonzales Chaves Macarena, Somoza Julia C, Friedman Silvia M, Zeni Susana N

机构信息

Oral and General Biochemistry Department, School of Dentistry, University of Buenos Aires, Buenos Aires, Argentina.

出版信息

J Periodontol. 2008 Jan;79(1):158-65. doi: 10.1902/jop.2008.070168.

DOI:10.1902/jop.2008.070168

PMID:18166106

Abstract

BACKGROUND

Previously, we measured bone alkaline phosphatase (b-ALP) and terminal C-telopeptide of collagen type I (CTX) in saliva. The present longitudinal experimental study sought to determine whether salivary concentrations of b-ALP and CTX have the same response as in serum samples under different conditions: normal, increased, and reduced bone remodeling.

METHODS

Thirty rats were ovariectomized (OVX) to induce osteopenia 60 days after surgery, and 10 rats were sham operated. Then, the rats were divided into four groups and treated as follows for 45 days: group 1 (G1) = SHAM + vehicle; group 2 (G2) = OVX + 8 microg olpadronate (OPD)/100 g of body weight; group 3 (G3) = OVX + 4 microg OPD/100 g of body weight; and group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T(75)). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days.

RESULTS

SHAM baseline and T(75) salivary b-ALP and CTX levels correlated with serum concentrations (P <0.01 and P <0.004, respectively). A correlation was observed between saliva and serum concentrations of b-ALP and CTX in OVX at baseline (P <0.0001 and P <0.004, respectively). Baseline salivary b-ALP and CTX levels were lower in SHAM animals compared to OVX groups (P <0.01). After treatment, T(75) saliva and serum CTX remained higher in G4 compared to G1 (P <0.05), was lower in G2 than in G1 (P <0.01) and G3 (P <0.01), and was similar in G1 and G3. Changes in BMD were the result of variations in salivary CTX levels due to OPD treatment (P <0.05).

CONCLUSIONS

Saliva determinations may prove to be practical and reliable for the detection of systemic signs of increased bone remodeling, particularly in cases involving pediatric, obese, and elderly patients, and in screening large populations. Moreover, saliva CTX may be one of the best candidate markers to detect the activity and severity of periodontal disease.

摘要

背景

此前，我们检测了唾液中的骨碱性磷酸酶（b-ALP）和I型胶原C端肽（CTX）。本纵向实验研究旨在确定在不同条件下（正常、骨重塑增加和减少），唾液中b-ALP和CTX的浓度是否与血清样本有相同的反应。

方法

30只大鼠接受卵巢切除术（OVX）以在术后60天诱导骨质减少，10只大鼠接受假手术。然后，将大鼠分为四组并进行如下处理45天：组1（G1）=假手术+赋形剂；组2（G2）=OVX+8μg唑来膦酸（OPD）/100g体重；组3（G3）=OVX+4μg OPD/100g体重；组4（G4）=OVX+赋形剂。在60天（基线）和75天（T(75)）时测定唾液和血清中的CTX和b-ALP。在基线和105天时使用双能X线吸收法测定腰椎和胫骨近端的骨密度（BMD）。

结果

假手术组基线和T(75)时唾液b-ALP和CTX水平与血清浓度相关（分别为P<0.01和P<0.004）。OVX组基线时唾液和血清中b-ALP和CTX浓度之间存在相关性（分别为P<0.0001和P<0.004）。与OVX组相比，假手术组动物的基线唾液b-ALP和CTX水平较低（P<0.01）。治疗后，与G1组相比，G4组T(75)时唾液和血清CTX仍较高（P<0.05），G2组低于G1组（P<0.01）和G3组（P<0.01），G1组和G3组相似。BMD的变化是由于OPD治疗导致唾液CTX水平变化的结果（P<0.05）。