Song Li-jun, Wang Zhao-xia, Chi Bao-rong, Yang Gui-zhen
Department of Pediatrics, First Hospital of Jilin University, Changchun 130021, China.
Zhonghua Yi Xue Za Zhi. 2007 Oct 30;87(40):2865-7.
To study the regulatory effect of diammonium glycyrrhizinate (GL) on the Th1/Th2 deviation in bronchial asthma.
Thirty Wistar rats were injected intraperitoneally with antigen solution including ovalbumin (OVA), inactivated pertussis vaccine, and aluminum hydroxide for the purpose of sensitization. Fourteen days later OVA was sprayed 20 min everyday for a consecutive 5 days to evoke sensitization, thus models of asthma were established. Then the 30 rats were randomly divided into 3 equal groups: asthma model group, model+GL group, and model+DXM group. Another 10 rats were used as control group. All rats underwent tracheal intubation to perform the breathing curves. By the end of the experiment the rats were killed with their lungs taken out to undergo pathological examination. Suspension of splenocytes was prepared, the level of interferon (IFN)-gamma, a cytokine mainly secreted by Th1 cells, was detected by L929-VSV system, and the IL-4 level was detected by ELISA.
The amplitude of the breathing curve of the GL group was similar to that of the DXM group, both significantly lower than that of the model group [(0.139+/-0.011) mV, both P<0.01] and higher than that of the control group. The respiratory rates of all groups became faster than normal. Pathology showed infiltration of inflammatory cells and proliferation of cup-shaped cells in the pneumonic tissues in the model group, and such damages were much slighter in the GL and DXM groups. The IFN-gamma level of the model group was (1.6+/-0.4) U, significantly lower than that of the control group [(3.7+/-1.0) U, P<0.05]. The IFN-gamma level of the GL group was (18.7+/-5.5) U, significantly higher than those of the DXM group [(2.1+/-0.3) U], model group and control group (all P<0.01). The IL-4 level of the model group was (305+/-36) pg, significantly higher than those of the GL group [(168+/-10) pg, P<0.01], DXM group [(213+/-47) pg], and control group [(54+/-6) pg, P<0.01], however, there was no significant difference between the GL and DXM groups.
GL increases the IFN-gamma level and decreases the IL-4 level, thus promoting the polarization of Th1 cells, regulating the Th1/Th2 deviation, and alleviating asthma.
研究甘草酸二铵（GL）对支气管哮喘Th1/Th2偏移的调节作用。
30只Wistar大鼠腹腔注射含卵清蛋白（OVA）、百日咳灭活疫苗和氢氧化铝的抗原溶液进行致敏。14天后，每天雾化吸入OVA 20分钟，连续5天激发致敏，建立哮喘模型。然后将30只大鼠随机分为3组，每组10只：哮喘模型组、模型+GL组、模型+地塞米松（DXM）组。另取10只大鼠作为对照组。所有大鼠行气管插管记录呼吸曲线。实验结束后处死大鼠，取肺组织进行病理检查。制备脾细胞悬液，采用L929-VSV系统检测Th1细胞主要分泌的细胞因子γ干扰素（IFN）-γ水平，采用酶联免疫吸附测定法（ELISA）检测白细胞介素（IL）-4水平。
GL组呼吸曲线幅度与DXM组相似，均显著低于模型组[（0.139±0.011）mV，P均<0.01]，高于对照组。各组呼吸频率均较正常加快。病理检查显示，模型组肺组织有炎性细胞浸润和杯状细胞增生，GL组和DXM组上述损伤较轻。模型组IFN-γ水平为（1.6±0.4）U，显著低于对照组[（3.7±1.0）U，P<0.05]。GL组IFN-γ水平为（18.7±5.5）U，显著高于DXM组[（2.1±0.3）U]、模型组和对照组（P均<0.01）。模型组IL-4水平为（305±36）pg，显著高于GL组[（168±10）pg，P<0.01]、DXM组[（213±47）pg]和对照组[（54±6）pg，P<0.01]，而GL组与DXM组比较差异无统计学意义。
GL可提高IFN-γ水平，降低IL-4水平，促进Th1细胞极化，调节Th1/Th2偏移，从而减轻哮喘。
