Shearan P, O'Keeffe M, Smyth M R
The National Food Centre, Teagasc Dunsinea, Castleknock, Dublin, Ireland.
Analyst. 1991 Dec;116(12):1365-8. doi: 10.1039/an9911601365.
A sensitive and selective high-performance liquid chromatographic procedure is described for the determination of the synthetic corticosteroid dexamethasone (DXM), in bovine muscle, kidney, liver and fat tissues, using methylprednisolone as the internal standard. Following extraction with ethyl acetate (muscle, kidney and liver) or diethyl ether (fat) and clean-up of the tissue extract, the drug residue was isolated using a C18 solid-phase extraction column. Separation of DXM was achieved by reversed-phase high-performance liquid chromatography with ultraviolet detection at 254 nm. By using this procedure, DXM levels as low as 0.01 mg kg-1 can be detected in muscle, kidney, liver and fat.
描述了一种灵敏且选择性高的高效液相色谱法，用于测定牛肌肉、肾脏、肝脏和脂肪组织中的合成皮质类固醇地塞米松（DXM），以内标甲泼尼龙作为对照。用乙酸乙酯（肌肉、肾脏和肝脏）或乙醚（脂肪）萃取，再对组织提取物进行净化处理，然后使用C18固相萃取柱分离药物残留。通过反相高效液相色谱法在254nm处进行紫外检测来分离DXM。采用此方法，可检测到肌肉、肾脏、肝脏和脂肪中低至0.01mg kg-1的DXM含量。
