Pascual Liliana M, Daniele María B, Giordano Walter, Pájaro María C, Barberis Isabel L
Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, 5800, Río Cuarto, Córdoba, Argentina.
Curr Microbiol. 2008 Apr;56(4):397-402. doi: 10.1007/s00284-007-9094-4. Epub 2008 Jan 3.
Lactobacillus fermentum strain L23 produced a small bacteriocin, designated bacteriocin L23, with an estimated molecular mass of < 7000 Da. Isolation, purification, and partial characterization of bacteriocin L23 are described. It displayed a wide inhibitory spectrum including both Gram-negative and Gram-positive pathogenic strains and two species of Candida. The antibacterial activity of cell-free culture supernatant fluid was not affected by catalase or urease but was abolished by the proteolytic enzymes trypsin and protease VI. Bacteriocin L23 was heat stable (60 min at 100 degrees C) and showed inhibitory activity over a wide pH range (4.0 to 7.0). The proteinaceous compound was isolated from cell-free culture supernatant fluid and purified. Crude bacteriocin sample was prepared by a process of ammonium sulfate precipitation, gel filtration, thin-layer chromatography, bioautography, and reversed-phase HPLC.
发酵乳杆菌L23菌株产生了一种小细菌素，命名为细菌素L23，估计分子量小于7000 Da。本文描述了细菌素L23的分离、纯化及部分特性。它具有广泛的抑制谱，包括革兰氏阴性和革兰氏阳性致病菌株以及两种念珠菌。无细胞培养上清液的抗菌活性不受过氧化氢酶或脲酶的影响，但被蛋白水解酶胰蛋白酶和蛋白酶VI消除。细菌素L23具有热稳定性（100℃ 60分钟），并在较宽的pH范围内（4.0至7.0）显示出抑制活性。从无细胞培养上清液中分离并纯化了这种蛋白质化合物。通过硫酸铵沉淀、凝胶过滤、薄层色谱、生物自显影和反相高效液相色谱法制备粗细菌素样品。
