Caetano Cátia Filipa, Gaspar Carlos, Oliveira Ana Sofia, Palmeira-de-Oliveira Rita, Rodrigues Lisa, Gonçalves Teresa, Martinez-de-Oliveira José, Palmeira-de-Oliveira Ana, Rolo Joana
CICS-UBI-Health Sciences Research Center, Faculty of Health Sciences, University of Beira Interior, 6200-506 Covilhã, Portugal.
Faculty of Health Sciences, University of Beira Interior, 6200-506 Covilhã, Portugal.
Microorganisms. 2023 Sep 26;11(10):2398. doi: 10.3390/microorganisms11102398.
The role of the fungal community, the mycobiota, in the health of the vagina is currently an important area of research. The emergence of new sequencing technologies and advances in bioinformatics made possible the discovery of novel fungi inhabiting this niche. spp. constitutes the most important group of opportunistic pathogenic fungi, being the most prevalent fungal species in vulvovaginal infections. However, fungi such as spp., spp. and spp. have emerged as potential pathogens in this niche, and therefore it is clinically relevant to understand their ecological interaction with spp. The main aim of this study was to evaluate the impact of yeasts on ' pathogenicity, focusing on in-vitro growth, and biofilm formation at different times of co-culture and germ tube formation. The assays were performed with isolated species or with co-cultures of (ATCC10231) with one other yeast species: (DSM13621), (DSM6170) or (DSM70215). The results showed that creates a symbiotic relationship with , enhancing the growth rate of the co-culture (149.69%), and of germ tube formation of (119.8%) and inducing a higher amount of biofilm biomass of the co-culture, both when mixed (154.1%) and preformed (166.8%). As for the yeasts and , the relationship is antagonistic (with a significant decrease in all assays), thus possibly repressing the mixture's pathogenicity. These results shed light on the complex interactions between yeasts in the vaginal mycobiome.
真菌群落，即真菌微生物群，在阴道健康中的作用是目前一个重要的研究领域。新测序技术的出现和生物信息学的进展使得发现栖息于这一生态位的新型真菌成为可能。白色念珠菌属构成了最重要的机会性致病真菌群体，是外阴阴道感染中最常见的真菌物种。然而，诸如光滑念珠菌属、热带念珠菌属和近平滑念珠菌属等真菌已成为这一生态位中的潜在病原体，因此了解它们与白色念珠菌属的生态相互作用具有临床相关性。本研究的主要目的是评估酵母菌对白色念珠菌属致病性的影响，重点关注共培养不同时间的体外生长、生物膜形成以及芽管形成。实验使用分离出的菌种或白色念珠菌（ATCC10231）与另一种酵母菌的共培养物进行：季也蒙毕赤酵母（DSM13621）、热带念珠菌（DSM6170）或近平滑念珠菌（DSM70215）。结果表明，季也蒙毕赤酵母与白色念珠菌建立了共生关系，提高了共培养物的生长速率（149.69%）、白色念珠菌的芽管形成率（119.8%），并在混合（154.1%）和预先形成（166.8%）时诱导共培养物产生更高量的生物膜生物量。至于酵母菌季也蒙毕赤酵母和热带念珠菌，它们的关系是拮抗的（在所有实验中均显著下降），因此可能抑制混合物的致病性。这些结果揭示了阴道真菌微生物群中酵母菌之间复杂的相互作用。
