Godlewska Renata, Pawlowski Marcin, Dzwonek Artur, Mikula Michal, Ostrowski Jerzy, Drela Nadzieja, Jagusztyn-Krynicka Elzbieta K
Department of Bacterial Genetics, Institute of Microbiology, University of Warsaw, ul. Miecznikowa 1, 02-096 Warsaw, Poland.
Curr Microbiol. 2008 Mar;56(3):279-86. doi: 10.1007/s00284-007-9083-7. Epub 2008 Jan 3.
A product of the Helicobacter pylori hp0596 gene (Tip-alpha) is a highly immunogenic homodimeric protein, unique for this bacterium. Cell fractionation experiments indicate that Tip-alpha is anchored to the inner membrane. In contrast, the three-dimensional model of the protein suggests that Tip-alpha is soluble or, at least, largely exposed to the solvent. hp0596 gene knockout resulted in a significant decrease in the level of H. pylori colonization as measured by real-time PCR assay. In addition, the Tip-alpha recombinant protein was determined to stimulate macrophage to produce IL-1alpha and TNF-alpha. Both results imply that Tip-alpha is rather loosely connected to the inner membrane and potentially released during infection.
幽门螺杆菌hp0596基因的产物（Tip-α）是一种高度免疫原性的同二聚体蛋白，这在该细菌中是独特的。细胞分级分离实验表明Tip-α锚定在内膜上。相比之下，该蛋白的三维模型表明Tip-α是可溶的，或者至少在很大程度上暴露于溶剂中。通过实时PCR测定法测量，hp0596基因敲除导致幽门螺杆菌定植水平显著降低。此外，已确定Tip-α重组蛋白可刺激巨噬细胞产生IL-1α和TNF-α。这两个结果都表明Tip-α与内膜的连接相当松散，并可能在感染期间释放。
