Kiliç Selcuk, Babür Cahit, Taylan Ozkan Ayşegül
Refik Saydam Hifzissihha Merkezi Başkanliği, Salgin Hastalikiar Araştirma Müdürlüğü, Parazitoloji Laboratuvari, Ankara.
Mikrobiyol Bul. 2007 Oct;41(4):571-7.
The aim of this retrospective study was to compare the results of indirect hemagglutination (IHA) and ELISA-IgG methods for the patients who were prediagnosed as hydatid cyst disease. Serum samples of 487 cases (301 female, 186 male; age range: 14-69 years) which have been sent to the Parasitology Laboratory of Refik Saydam Hygiene Center between the period of 2003 to 2005 were included to the study. Echinococcus granulosus specific antibodies were searched by commercial IHA (Fumouse Laboratories, France) and ELISA-IgG (r-Biopharm, Germany) kits. Of the serum samples, 284 (58.3%) were found negative, and 173 (35.5%) were found positive with both of the methods. Nineteen samples (3.9%) which were detected as negative by IHA, were found positive by ELISA. In 11 samples (2.3%) which were found negative by ELISA, agglutination reaction were observed at the titers of 1/160-1/1280 by IHA. The results were evaluated by Kappa statistical analysis with 95% confidence intervals and the results of IHA and ELISA tests showed high correlation (kappa = 0.88) by means of the serological diagnosis of hydatid disease. In evaluation of the cases whose sera were positive with both of the methods, no statistical differences was observed in terms of gender (chi2 = 0.08, p = 0.78), and the highest seropositivity (43.4%) rate was observed in the 40-56 years age group. As a result, although our data indicated a high correlation between ELISA and IHA methods, the use of these tests together will be favorable for the screening of suspected patients in terms of comparability and confidency, in routine laboratories which do not have economical limitations.
本回顾性研究的目的是比较间接血凝试验（IHA）和ELISA-IgG方法对预先诊断为包虫囊肿病患者的检测结果。纳入了2003年至2005年期间送至Refik Saydam卫生中心寄生虫学实验室的487例患者的血清样本（301例女性，186例男性；年龄范围：14 - 69岁）。使用商业化的IHA试剂盒（法国Fumouse Laboratories公司）和ELISA-IgG试剂盒（德国r-Biopharm公司）检测细粒棘球绦虫特异性抗体。在血清样本中，284份（58.3%）两种方法检测均为阴性，173份（35.5%）两种方法检测均为阳性。IHA检测为阴性的19份样本（3.9%），ELISA检测为阳性。ELISA检测为阴性的11份样本（2.3%），IHA检测在1/160 - 1/1280滴度出现凝集反应。通过Kappa统计分析及95%置信区间评估结果，IHA和ELISA检测结果在包虫病血清学诊断方面显示出高度相关性（kappa = 0.88）。在评估两种方法血清均为阳性的病例时，性别方面未观察到统计学差异（χ2 = 0.08，p = 0.78），40 - 56岁年龄组血清阳性率最高（43.4%）。结果表明，尽管我们的数据显示ELISA和IHA方法之间具有高度相关性，但在没有经济限制的常规实验室中，将这些检测方法联合使用，在可比性和可信度方面对疑似患者的筛查将更有利。
