Jahani Zahra, Meshgi Behnam, Rajabi-Bzl Masomeh, Jalousian Fatemeh, Hasheminasab Sajad
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Dept. of Medical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Parasitol. 2014 Apr-Jun;9(2):218-25.
Cystic echinococcosis caused by the metacestode of Echinococcus granulosus is a major problem in both humans and domestic animals health. Therefore, a standardized and approachable diagnostic tool (rapid tests) for the serodiagnosis of cystic echinococcosis (CE) is still needed.
In the present work, antigen B labeled with gold nanoparticles was used to detect antibodies against hydatid cyst disease. The prepared antigen B was analyzed by SDS-PAGE. Tetra chloroauric acid (HAuCl4) was used to produce colloidal gold and antigen B labeled by gold nanoparticles, then it was tested by using rabbits antisera and sera from naturally infected sheep. The labeled antigen B was evaluated using Dot-immunogold staining (Dot-IGS) method.
Electrophoretic pattern of hydatid cyst fluid showed the quality of bands in the condensed fluid is better than crude fluid. SDS-PAGE analysis cyst fluid and antigen B revealed three specific protein bands that were detected at molecular weights of 24, 30 and 40 kDa that all are the subunits of antigen B. Evaluation of antigen B labeled by gold nanoparticles by using Dot-IGS technique showed 1/1 and 1/50 dilutions in comparison with another has the best immunoreaction. In this method, nanoparticles produced a typical purple color, when they binded to the strip at the site of immunoreaction.
Therefore, using gold nanoparticles is a good candidate for detection of helminthiasis, also as selective tools of early detection, simple and cost-effective, regardless of specific skills and equipment with optimal durability.
由细粒棘球绦虫的幼虫引起的囊型包虫病是人类和家畜健康的主要问题。因此，仍然需要一种标准化且易于使用的诊断工具（快速检测）用于囊型包虫病（CE）的血清学诊断。
在本研究中，用金纳米颗粒标记的抗原B来检测抗包虫囊肿病的抗体。通过SDS-PAGE分析制备的抗原B。用氯金酸（HAuCl4）制备胶体金并标记抗原B，然后用兔抗血清和自然感染绵羊的血清进行检测。用斑点免疫金染色（Dot-IGS）法评估标记的抗原B。
包虫囊肿液的电泳图谱显示浓缩液中的条带质量优于粗液。SDS-PAGE分析囊肿液和抗原B显示在分子量为24、30和40 kDa处检测到三条特异性蛋白带，这些都是抗原B的亚基。用Dot-IGS技术评估金纳米颗粒标记的抗原B，结果显示1/1和1/50稀释度与另一种稀释度相比具有最佳免疫反应。在该方法中，纳米颗粒在免疫反应部位与试纸条结合时产生典型的紫色。
因此，使用金纳米颗粒是检测蠕虫病的良好候选方法，也是早期检测的选择性工具，简单且经济高效，无需特定技能和设备，且具有最佳耐用性。
