Yalinkilic Omer, Enginar Huseyin
Kumluca Anatolian Vocation High School, Kumluca, Antalya, Turkey.
Photochem Photobiol. 2008 Jan-Feb;84(1):236-42. doi: 10.1111/j.1751-1097.2007.00240.x.
The aim of this study was to investigate the effect of three saponin-containing plant species extracts (Aesculuc hippocastanum L. seed extract [AHE], Medicago sativa L. extract [MSE] and Spinacia oleracea L. extract [SOE]) on lipid peroxidation and on antioxidant systems in rats exposed to X-rays (XR). The rats were divided into three categories. The first category served as controls and received only a standard diet. The second category served as the radiation group and received 5 and 10 Gy XR dose. The third category (XR+extract-treated) received plant extracts (25.0 or 50.0 mg kg(-1) live weight) and 5 or 10 Gy XR dose. Blood samples were analyzed for their content of malondialdehyde (MDA), reduced glutathione (GSH), plasma vitamin C, beta-carotene and retinol. In animals receiving XR, the plasma MDA (P < 0.001) value significantly increased but the level of GSH (P < 0.01), vitamin C (P < 0.001), retinol and beta-carotene (P < 0.001) decreased significantly with increasing XR doses. In the XR+extract-treated groups, the concentrations of MDA increased significantly with increasing radiation but their concentrations decreased significantly with increasing extract concentrations. Plasma concentrations of GSH, beta-carotene, retinol and vitamin C in XR+extract-treated groups decreased significantly with increasing XR dose but their concentrations increased with increasing extract doses. Further, comparison of blood samples of XR+extract-treated groups with those from the control group showed that GSH, beta-carotene, retinol and vitamin C values increased significantly but that MDA values decreased significantly. The results showed that all extracts have enhanced the antioxidant status and decreased the incidence of free radical-induced lipid peroxidation in blood samples of rats exposed to XR. However, the antioxidant effect of AHE-administered animals was more effective than that of MSE- and SOE-administered whole-body XR rats. We conclude that the supplementation with saponin-containing extracts may serve to reinforce the antioxidant systems, thus having protective effect against cell damage by XR.
本研究的目的是调查三种含皂苷植物提取物（欧洲七叶树种子提取物[AHE]、紫花苜蓿提取物[MSE]和菠菜提取物[SOE]）对接受X射线（XR）照射的大鼠脂质过氧化和抗氧化系统的影响。大鼠分为三类。第一类作为对照组，仅接受标准饮食。第二类作为辐射组，接受5 Gy和10 Gy的XR剂量。第三类（XR+提取物处理组）接受植物提取物（25.0或50.0 mg kg⁻¹体重）和5或10 Gy的XR剂量。分析血样中丙二醛（MDA）、还原型谷胱甘肽（GSH）、血浆维生素C、β-胡萝卜素和视黄醇的含量。在接受XR的动物中，随着XR剂量增加，血浆MDA值显著升高（P < 0.001），但GSH水平（P < 0.01）、维生素C（P < 0.001）、视黄醇和β-胡萝卜素（P < 0.001）显著降低。在XR+提取物处理组中，MDA浓度随辐射增加而显著升高，但随提取物浓度增加而显著降低。XR+提取物处理组中GSH、β-胡萝卜素、视黄醇和维生素C的血浆浓度随XR剂量增加而显著降低，但随提取物剂量增加而升高。此外，将XR+提取物处理组的血样与对照组的血样进行比较，结果显示GSH、β-胡萝卜素、视黄醇和维生素C值显著升高，但MDA值显著降低。结果表明，所有提取物均增强了抗氧化状态，并降低了接受XR照射的大鼠血样中自由基诱导的脂质过氧化发生率。然而，给予AHE的动物的抗氧化作用比给予MSE和SOE的全身XR大鼠更有效。我们得出结论，补充含皂苷提取物可能有助于增强抗氧化系统，从而对XR引起的细胞损伤具有保护作用。
