Levanti M B, Montalbano G, Laurà R, Ciriaco E, Cobo T, García-Suarez O, Germanà A, Vega J A
Dipartmento di Morfologia, Biochimica, Fisiologia e Produzione Animale, Sezione di Morfologia, Facoltà di Medicina Veterinaria, Università di Messina, Italy.
J Anat. 2008 Jan;212(1):67-71. doi: 10.1111/j.1469-7580.2007.00836.x.
Calretinin is a calcium-binding protein found widely distributed in the central nervous system and chemosensory cells of the teleosts, but its presence in the peripheral nervous system of fishes is unknown. In this study we used Western blot analysis and immunohistochemistry to investigate the occurrence and distribution of calretinin in the cranial nerve ganglia, dorsal root ganglia, sympathetic ganglia, and enteric nervous system of the adult zebrafish. By Western blotting a unique and specific protein band with an estimated molecular weight of around 30 kDa was detected, and it was identified as calretinin. Immunohistochemistry revealed that calretinin is selectively present in the cytoplasm of the neurons and never in the satellite glial cells. In both sensory and sympathetic ganglia the density of neurons that were immunolabelled, their size and morphology, as well as the intensity of immunostaining developed within the cytoplasm, were heterogeneous. In the enteric nervous system calretinin immunoreactivity was detected in a subset of enteric neurons as well as in a nerve fibre plexus localized inside the muscular layers. The present results demonstrate that in addition to the central nervous system, calretinin is also present in the peripheral nervous system of zebrafish, and contribute to completing the map of the distribution of this protein in the nervous system of teleosts.
钙视网膜蛋白是一种钙结合蛋白，广泛分布于硬骨鱼的中枢神经系统和化学感受细胞中，但它在鱼类外周神经系统中的存在情况尚不清楚。在本研究中，我们使用蛋白质免疫印迹分析和免疫组织化学方法，研究成年斑马鱼的脑神经节、背根神经节、交感神经节和肠神经系统中钙视网膜蛋白的存在情况和分布。通过蛋白质免疫印迹分析，检测到一条独特的特异性蛋白条带，估计分子量约为30 kDa，并将其鉴定为钙视网膜蛋白。免疫组织化学显示，钙视网膜蛋白选择性地存在于神经元的细胞质中，而在卫星神经胶质细胞中从未检测到。在感觉神经节和交感神经节中，免疫标记的神经元密度、大小和形态，以及细胞质内免疫染色的强度都是异质性的。在肠神经系统中，在一部分肠神经元以及位于肌肉层内的神经纤维丛中检测到了钙视网膜蛋白免疫反应性。目前的结果表明，除了中枢神经系统外，钙视网膜蛋白也存在于斑马鱼的外周神经系统中，有助于完善该蛋白在硬骨鱼神经系统中的分布图。
