Lieschke Graham J, Currie Peter D
Cancer and Haematology Division, Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria, 3050, Australia.
Nat Rev Genet. 2007 May;8(5):353-67. doi: 10.1038/nrg2091.
Despite the pre-eminence of the mouse in modelling human disease, several aspects of murine biology limit its routine use in large-scale genetic and therapeutic screening. Many researchers who are interested in an embryologically and genetically tractable disease model have now turned to zebrafish. Zebrafish biology allows ready access to all developmental stages, and the optical clarity of embryos and larvae allow real-time imaging of developing pathologies. Sophisticated mutagenesis and screening strategies on a large scale, and with an economy that is not possible in other vertebrate systems, have generated zebrafish models of a wide variety of human diseases. This Review surveys the achievements and potential of zebrafish for modelling human diseases and for drug discovery and development.
尽管小鼠在人类疾病建模方面占据主导地位，但小鼠生物学的几个方面限制了它在大规模基因和治疗筛选中的常规应用。许多对胚胎学和遗传学上易于操作的疾病模型感兴趣的研究人员现在已转向斑马鱼。斑马鱼生物学特性使得能够轻易获取所有发育阶段，并且胚胎和幼虫的光学透明度允许对发育中的病理情况进行实时成像。大规模且具有其他脊椎动物系统所没有的经济性的复杂诱变和筛选策略，已产生了多种人类疾病的斑马鱼模型。本综述探讨了斑马鱼在人类疾病建模以及药物发现与开发方面的成就和潜力。
