Arco A, Faure M, Piemont Y, Thivolet J
Dipartimento di Biomorfologia, Università di Messina.
Arch Ital Anat Embriol. 1991 Jul-Sep;96(3):185-99.
The toxic effects of the two serotypes of staphylococcal exotoxin: exfoliatin A (ETA) and B (ETB) on two experimental models: organotypic cultures of the skin and cellular cultures of the epidermis reconstructed "in vitro" have been studied. The results show that, in both cases, purified ETB (Fig. 4a, 6-6a, b, c) reproduces the characteristics of Lyell's Staphylococcal Syndrome that is the intraepidermal cleavage either between the granulosa and the spinous layers or at the granulosa layer of the epidermis. As the images of the LM and EM demonstrate, purified ETA behaves differently in the two experimental models; in fact, it produces the same effect as purified toxin B on the organotypic cultures (Fig. 3a, b), whereas it causes no alterations in the epithelial cultures reproduced "in vitro" (Fig. 5a, b).
剥脱毒素A（ETA）和B（ETB）对两种实验模型的毒性作用，这两种模型分别是皮肤器官型培养物和“体外”重建的表皮细胞培养物。结果表明，在这两种情况下，纯化的ETB（图4a、6 - 6a、b、c）再现了莱尔葡萄球菌综合征的特征，即在颗粒层和棘层之间或表皮颗粒层处的表皮内分离。正如光镜和电镜图像所示，纯化的ETA在两种实验模型中的表现不同；事实上，它在器官型培养物上产生与纯化毒素B相同的效果（图3a、b），而在“体外”重建的上皮细胞培养物中不引起任何改变（图5a、b）。
