两种免疫调节剂姜黄素和柳氮磺胺吡啶可增强茚地那韦在HIV-1持续感染细胞中的抗逆转录病毒活性。

Two immunomodulators, curcumin and sulfasalazine, enhance IDV antiretroviral activity in HIV-1 persistently infected cells.

作者信息

Riva D A, Fernández-Larrosa P N, Dolcini G L, Martínez-Peralta L A, Coulombié F C, Mersich S E

机构信息

Laboratory of Virology, Department of Biochemistry, School of Science, University of Buenos Aires, Int. Güiraldes 2160, Pab2 P4, Ciudad Universitaria, C1428EGA, Buenos Aires, Argentina.

出版信息

Arch Virol. 2008;153(3):561-5. doi: 10.1007/s00705-007-0023-4. Epub 2008 Jan 4.

DOI:10.1007/s00705-007-0023-4

PMID:18175040

Abstract

Since the appearance of resistance to antiretroviral treatment is unavoidable, the host cell's transcription factor NF-kappaB is a novel HIV target. The goal of this study was to characterize the effect of two immunomodulators, curcumin (Cur) and sulfasalazine (Sul), with a protease inhibitor, indinavir (IDV), on HIV-1 persistently infected CD4+ T-cells. Viral p24 antigen production, viral infectivity (tested on MAGI cells) and viral relative infectivity (viral infectivity/p24) were analysed. When used alone, both immunomodulators were able to reduce viral infectivity. When in combination, both 10 microM IDV plus 10 microM Cur and 10 microM IDV plus 250 microM Sul showed a significant reduction in viral infectivity and viral relative infectivity when compared to the reduction produced by IDV alone. Thus, the use of immunomodulators with IDV could help to reduce HIV-1 production in persistently infected cells.

摘要

由于对抗逆转录病毒治疗产生耐药性是不可避免的，宿主细胞的转录因子核因子κB（NF-κB）是一种新型的HIV靶点。本研究的目的是表征两种免疫调节剂姜黄素（Cur）和柳氮磺胺吡啶（Sul）与蛋白酶抑制剂茚地那韦（IDV）对HIV-1持续感染的CD4+T细胞的影响。分析了病毒p24抗原的产生、病毒感染性（在MAGI细胞上检测）和病毒相对感染性（病毒感染性/p24）。单独使用时，两种免疫调节剂都能够降低病毒感染性。联合使用时，与单独使用IDV相比，10微摩尔IDV加10微摩尔Cur和10微摩尔IDV加250微摩尔Sul均显示病毒感染性和病毒相对感染性显著降低。因此，将免疫调节剂与IDV联合使用有助于减少持续感染细胞中HIV-1的产生。