从日本海沿岸沙质沉积物中分离出的新属新种的鉴定与基因组分析
Characterization and Genomic Analysis of gen. nov., sp. nov., Isolated from the Sandy Sediments of the Sea of Japan Seashore.
作者信息
Romanenko Lyudmila, Otstavnykh Nadezhda, Tanaka Naoto, Kurilenko Valeriya, Svetashev Vasily, Tekutyeva Liudmila, Mikhailov Valery, Isaeva Marina
机构信息
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok 690022, Russia.
NODAI Culture Collection Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
出版信息
Microorganisms. 2023 Sep 25;11(10):2385. doi: 10.3390/microorganisms11102385.
The taxonomic status of two gram-negative, whitish-pigmented motile bacteria KMM 9576 and KMM 9553 isolated from a sandy sediment sample from the Sea of Japan seashore was defined. Phylogenetic analysis revealed that strains KMM 9576 and KMM 9553 represent a distinct lineage within the family sharing 100% 16S rRNA sequence similarity and 99.5% average nucleotide identity (ANI) to each other. The strains showed the highest 16S rRNA sequence similarities of 97.4% to LMG 24692, 96.9% to NBRC 100388, and 96.8% to NBRC 107135. The ANI values between strain KMM 9576 and NBRC 100388, USDA 205, NBRC 107135, and NBRC 14778 were 79.9%, 79.6%, 79.4%, and 79.2%, respectively. The highest core-proteome average amino acid identity (cpAAI) values of 82.1% and 83.1% were estimated between strain KMM 9576 and NBRC 14778 and '' NS-104, respectively. The DNA GC contents were calculated from a genome sequence to be 61.5% (KMM 9576) and 61.4% (KMM 9553). Both strains contained the major ubiquinone Q-10 and C7 as the dominant fatty acid followed by 11-methyl C7 and C cyclo, and polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, and two unidentified phospholipids. Based on phylogenetic and phylogenomic analyses, and phenotypic characterization, strains KMM 9576 and KMM 9553 are concluded to represent a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is strain KMM 9576 (=NRIC 0957).
确定了从日本海沿岸沙质沉积物样本中分离出的两株革兰氏阴性、白色色素沉着的运动细菌KMM 9576和KMM 9553的分类地位。系统发育分析表明，菌株KMM 9576和KMM 9553代表了该科内一个独特的谱系，它们的16S rRNA序列相似性为100%，平均核苷酸同一性（ANI）为99.5%。这些菌株与LMG 24692的16S rRNA序列相似性最高，为97.4%，与NBRC 100388的相似性为96.9%，与NBRC 107135的相似性为96.8%。菌株KMM 9576与NBRC 100388、USDA 205、NBRC 107135和NBRC 14778之间的ANI值分别为79.9%、79.6%、79.4%和79.2%。菌株KMM 9576与NBRC 14778和“NS-104”之间估计的最高核心蛋白质组平均氨基酸同一性（cpAAI）值分别为82.1%和83.1%。根据基因组序列计算，DNA的GC含量分别为61.5%（KMM 9576）和61.4%（KMM 9553）。两株菌都含有主要的泛醌Q-10和C7作为主要脂肪酸，其次是11-甲基C7和C环丙烷脂肪酸，极性脂质由磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的氨基磷脂和两种未鉴定的磷脂组成。基于系统发育和系统基因组分析以及表型特征，得出菌株KMM 9576和KMM 9553代表一个新属和新种的结论，并为此提议命名为新属，新种。新物种的模式菌株是菌株KMM 9576（=NRIC 0957）。
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