Albuquerque Luciana, Tiago Igor, Taborda Marco, Nobre M Fernanda, Veríssimo António, da Costa Milton S
Centro de Neurociências e Biologia Celular, Department of Zoology, University of Coimbra, 3004-517 Coimbra, Portugal.
Int J Syst Evol Microbiol. 2008 Jan;58(Pt 1):226-30. doi: 10.1099/ijs.0.65217-0.
A low-G+C, Gram-positive isolate, designated strain CVS-8(T), was isolated from a sea salt evaporation pond on the Island of Sal in the Cape Verde Archipelago. This organism was found to be a catalase- and oxidase-positive, non-motile, spore-forming, aerobic, curved rod-shaped organism with an optimum growth temperature of about 35-37 degrees C and an optimum pH between 7.5 and 8.0. Optimal growth occurred in media containing 4-6% (w/v) NaCl and no growth occurred in medium without NaCl. The cell-wall peptidoglycan was of the A1gamma type with meso-diaminopimelic acid, the major respiratory quinone was MK-7, the major fatty acids were iso-15:0, 16:0, anteiso-15:0 and iso-16:0 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminoglycophospholipid. The G+C content of the DNA was 37.9 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain CVS-8(T) represented a novel species of the genus Bacillus, the highest levels of sequence similarity (mean pairwise similarity values of approximately 97.5 %) being found with respect to the type strains of Bacillus shackletonii and Bacillus acidicola. On the basis of the phylogenetic, physiological and biochemical data, strain CVS-8(T) represents a novel species of the genus Bacillus, for which the name Bacillus isabeliae sp. nov. is proposed. The type strain is CVS-8(T) (=LMG 22838(T)=CIP 108578(T)).
从佛得角群岛萨尔岛的一个海盐蒸发池中分离出一株低G+C含量的革兰氏阳性菌，命名为菌株CVS-8(T)。该菌株为过氧化氢酶和氧化酶阳性、不运动、产芽孢、需氧的弯曲杆状菌，最适生长温度约为35-37℃，最适pH值在7.5至8.0之间。在含有4-6%（w/v）NaCl的培养基中生长最佳，在不含NaCl的培养基中不生长。细胞壁肽聚糖为含内消旋二氨基庚二酸的A1γ型，主要呼吸醌为MK-7，主要脂肪酸为异十五烷酸、十六烷酸、anteiso-十五烷酸和异十六烷酸，主要极性脂为二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和一种未鉴定的氨基糖磷脂。DNA的G+C含量为37.9 mol%。16S rRNA基因序列的系统发育分析表明，菌株CVS-8(T)代表芽孢杆菌属的一个新物种，与沙克尔顿芽孢杆菌和嗜酸芽孢杆菌的模式菌株的序列相似性最高（平均成对相似性值约为97.5%）。根据系统发育、生理和生化数据，菌株CVS-8(T)代表芽孢杆菌属的一个新物种，为此提出新名称伊莎贝拉芽孢杆菌（Bacillus isabeliae sp. nov.）。模式菌株为CVS-8(T)（=LMG 22838(T)=CIP 108578(T)）。
