Liu Huan, Zhou Yu, Liu Rui, Zhang Ke-Yun, Lai Ren
Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, PR China.
Int J Syst Evol Microbiol. 2009 Jun;59(Pt 6):1460-4. doi: 10.1099/ijs.0.000653-0.
A novel Gram-positive, motile, rod-shaped bacterium isolated from a saline soil in China was characterized by a polyphasic taxonomic approach. The strain, designated YC1(T), was halotolerant [tolerating up to 15 % (w/v) NaCl] and alkaliphilic (growing at a broad pH range of 5-13). 16S rRNA gene sequence analysis revealed that the isolate belonged to the genus Bacillus, showing highest similarity to Bacillus macauensis JCM 13285(T) (98.0 %). However, DNA-DNA hybridization indicated low levels of genomic relatedness with B. macauensis JCM 13285(T) (8.5 %). The major isoprenoid quinone was MK-7 and the cellular fatty acid profile consisted of significant amounts of iso-C(15 : 0) (38.6 %) and anteiso-C(15 : 0) (35.9 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The G+C content of the genomic DNA was 41.8 mol%. On the basis of the polyphasic evidence from this study, strain YC1(T) (=KCTC 13181(T)=CGMCC 1.6854(T)) should be classified as the type strain of a novel species of the genus Bacillus, for which the name Bacillus solisalsi sp. nov. is proposed.
采用多相分类法对从中国盐碱土中分离出的一种新型革兰氏阳性、具运动性的杆状细菌进行了表征。该菌株命名为YC1(T)，具有耐盐性[可耐受高达15%（w/v）的NaCl]和嗜碱性（在5 - 13的广泛pH范围内生长）。16S rRNA基因序列分析表明，该分离株属于芽孢杆菌属，与澳门芽孢杆菌JCM 13285(T)的相似性最高（98.0%）。然而，DNA - DNA杂交显示与澳门芽孢杆菌JCM 13285(T)的基因组相关性较低（8.5%）。主要类异戊二烯醌为MK - 7，细胞脂肪酸谱由大量的异-C(15 : 0)（38.6%）和anteiso - C(15 : 0)（35.9%）组成。主要极性脂为双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。基因组DNA的G + C含量为41.8 mol%。基于本研究的多相证据，菌株YC1(T)（=KCTC 13181(T)=CGMCC 1.6854(T)）应被分类为芽孢杆菌属一个新物种的模式菌株，为此提出芽孢杆菌盐碱土种（Bacillus solisalsi sp. nov.）这一名称。
