Lebedeva E V, Alawi M, Maixner F, Jozsa P-G, Daims H, Spieck E
Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Oktyabrya 7/2, Moscow 117312, Russia.
Int J Syst Evol Microbiol. 2008 Jan;58(Pt 1):242-50. doi: 10.1099/ijs.0.65379-0.
A new isolate of a lithoautotrophic nitrite-oxidizing bacterium was obtained from internal corrosion deposits from a steel pipeline of the Moscow heating system. The organism oxidized nitrite as the sole energy source and fixed carbon dioxide as the only carbon source. The cells were extremely pleomorphic: loosely wound spirals, slightly curved and even straight rods were detected, as well as coccoid cells. The highest rate of nitrite consumption (1.5 mM nitrite as substrate) was measured at 42 degrees C, with a temperature range of 28-44 degrees C. In enrichment cultures with Nocardioides sp. as an accompanying organism, optimal oxidation of 5.8 mM nitrite occurred at 45 degrees C, with a range of 28-48 degrees C. Neither pyruvate nor yeast extract stimulated nitrification. Organotrophic growth was not observed. Phylogenetic analysis of 16S rRNA gene sequences revealed that the novel isolate represents a new sublineage of the genus Nitrospira. On the basis of physiological, chemotaxonomic and molecular characteristics, the name 'Candidatus Nitrospira bockiana' is proposed.
从莫斯科供热系统的一条钢管内部腐蚀沉积物中获得了一种新的化能自养亚硝酸盐氧化细菌菌株。该微生物以亚硝酸盐作为唯一能源进行氧化，并以二氧化碳作为唯一碳源进行固定。细胞具有极其多样的形态：检测到松散缠绕的螺旋体、微弯甚至直杆状细胞，以及球状细胞。在42℃时测得亚硝酸盐消耗的最高速率（以1.5 mM亚硝酸盐为底物），温度范围为28 - 44℃。在与诺卡氏菌属（Nocardioides sp.）作为伴生菌的富集培养物中，5.8 mM亚硝酸盐的最佳氧化发生在45℃，范围为28 - 48℃。丙酮酸和酵母提取物均未刺激硝化作用。未观察到有机营养生长。对16S rRNA基因序列的系统发育分析表明，该新分离株代表硝化螺菌属（Nitrospira）的一个新亚谱系。基于生理、化学分类和分子特征，提出了“新鞘氨醇硝化螺菌（Candidatus Nitrospira bockiana）”这一名称。
